Journal article
In vitro mechanistic study towards a better understanding of ZnO nanoparticle toxicity.
- Buerki-Thurnherr T Empa, Swiss Federal Laboratories for Materials Testing and Technology, Laboratory for Materials-Biology Interactions, Lerchenfeldstrasse, St. Gallen, Switzerland. tina.buerki@empa.ch
- Xiao L
- Diener L
- Arslan O
- Hirsch C
- Maeder-Althaus X
- Grieder K
- Wampfler B
- Mathur S
- Wick P
- Krug HF
- 2012-03-08
Published in:
- Nanotoxicology. - 2013
Caspases
Cell Death
Gene Expression Regulation, Enzymologic
Humans
Jurkat Cells
Membrane Potential, Mitochondrial
Metal Nanoparticles
Microscopy, Electron, Transmission
Reactive Oxygen Species
Zinc Oxide
English
ZnO nanoparticles (NPs) elicit significant adverse effects in various cell types, organisms and in the environment. The toxicity of nanoscale ZnO has often been ascribed to the release of zinc ions from the NPs but it is not yet understood to which extent these ions contribute to ZnO NP toxicity and what are the underlying mechanisms. Here, we take one step forward by demonstrating that ZnO-induced Jurkat cell death is largely an ionic effect involving the extracellular release of high amounts of Zn(II), their rapid uptake by the cells and the induction of a caspase-independent alternative apoptosis pathway that is independent of the formation of ROS. In addition, we identified novel coating strategies to reduce ZnO NP dissolution and subsequent adverse effects.
- Language
-
- English
- Open access status
- closed
- Identifiers
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- DOI 10.3109/17435390.2012.666575
- PMID 22394310
- Persistent URL
- https://sonar.ch/global/documents/112350
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