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GC content shapes mRNA storage and decay in human cells
Journal article

GC content shapes mRNA storage and decay in human cells

  • Courel, Maïté Sorbonne Université, CNRS, Institut de Biologie Paris Seine (IBPS), Laboratoire de Biologie du Développement, Paris, France
  • Clément, Yves ORCID Ecole Normale Supérieure, Institut de Biologie de l'ENS, IBENS, Paris, France
  • Bossevain, Clémentine Sorbonne Université, CNRS, Institut de Biologie Paris Seine (IBPS), Laboratoire de Biologie du Développement, Paris, France
  • Foretek, Dominika ncRNA, Epigenetic and Genome Fluidity, Institut Curie, PSL Research University, CNRS UMR 3244, Sorbonne Université, Paris, France
  • Vidal Cruchez, Olivia Université Côte d'Azur, CNRS, INSERM, IRCAN, FHU-OncoAge, Nice, France
  • Yi, Zhou Université Côte d'Azur, CNRS, INSERM, iBV, Nice, France
  • Bénard, Marianne Sorbonne Université, CNRS, Institut de Biologie Paris Seine (IBPS), Laboratoire de Biologie du Développement, Paris, France
  • Benassy, Marie-Noëlle Sorbonne Université, CNRS, Institut de Biologie Paris Seine (IBPS), Laboratoire de Biologie du Développement, Paris, France
  • Kress, Michel Sorbonne Université, CNRS, Institut de Biologie Paris Seine (IBPS), Laboratoire de Biologie du Développement, Paris, France
  • Vindry, Caroline Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom
  • Ernoult-Lange, Michèle Sorbonne Université, CNRS, Institut de Biologie Paris Seine (IBPS), Laboratoire de Biologie du Développement, Paris, France
  • Antoniewski, Christophe ORCID Sorbonne Université, CNRS, Institut de Biologie Paris Seine (IBPS), ARTbio Bioinformatics Analysis Facility, Paris, France
  • Morillon, Antonin ORCID ncRNA, Epigenetic and Genome Fluidity, Institut Curie, PSL Research University, CNRS UMR 3244, Sorbonne Université, Paris, France
  • Brest, Patrick Université Côte d'Azur, CNRS, INSERM, IRCAN, FHU-OncoAge, Nice, France
  • Hubstenberger, Arnaud Université Côte d'Azur, CNRS, INSERM, iBV, Nice, France
  • Roest Crollius, Hugues ORCID Ecole Normale Supérieure, Institut de Biologie de l'ENS, IBENS, Paris, France
  • Standart, Nancy Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom
  • Weil, Dominique ORCID Sorbonne Université, CNRS, Institut de Biologie Paris Seine (IBPS), Laboratoire de Biologie du Développement, Paris, France
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  • 2019-12-19
Published in:
  • eLife. - eLife Sciences Publications, Ltd. - 2019, vol. 8
General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology
General Neuroscience
General Medicine
English mRNA translation and decay appear often intimately linked although the rules of this interplay are poorly understood. In this study, we combined our recent P-body transcriptome with transcriptomes obtained following silencing of broadly acting mRNA decay and repression factors, and with available CLIP and related data. This revealed the central role of GC content in mRNA fate, in terms of P-body localization, mRNA translation and mRNA stability: P-bodies contain mostly AU-rich mRNAs, which have a particular codon usage associated with a low protein yield; AU-rich and GC-rich transcripts tend to follow distinct decay pathways; and the targets of sequence-specific RBPs and miRNAs are also biased in terms of GC content. Altogether, these results suggest an integrated view of post-transcriptional control in human cells where most translation regulation is dedicated to inefficiently translated AU-rich mRNAs, whereas control at the level of 5’ decay applies to optimally translated GC-rich mRNAs.
Language
  • English
Open access status
gold
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Persistent URL
https://sonar.ch/global/documents/112601
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