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Journal article
Production of highly polarized [1-13 C]acetate by rapid decarboxylation of [2-13 C]pyruvate - application to hyperpolarized cardiac spectroscopy and imaging.
- Steinhauser J Institute for Biomedical Engineering, University and ETH Zurich, Zurich, Switzerland.
- Wespi P Institute for Biomedical Engineering, University and ETH Zurich, Zurich, Switzerland.
- Kwiatkowski G Institute for Biomedical Engineering, University and ETH Zurich, Zurich, Switzerland.
- Kozerke S Institute for Biomedical Engineering, University and ETH Zurich, Zurich, Switzerland.
- 2019-05-03
Published in:
- Magnetic resonance in medicine. - 2019
DNP
acetate
cardiac metabolism
dynamic nuclear polarisation
hyperpolarized 13C
Acetates
Animals
Carbon Isotopes
Decarboxylation
Female
Heart
Image Processing, Computer-Assisted
Magnetic Resonance Imaging
Myocardium
Phantoms, Imaging
Pyruvic Acid
Rats
Rats, Sprague-Dawley
Signal Processing, Computer-Assisted
English
PURPOSE
The objective of the present work was to develop and implement an efficient approach to hyperpolarize [1-13 C]acetate and apply it to in vivo cardiac spectroscopy and imaging.
METHODS
Rapid hydrogen peroxide induced decarboxylation was used to convert hyperpolarized [2-13 C]pyruvate into highly polarized [1-13 C]acetate employing an additional step following rapid dissolution of [2-13 C]pyruvate in a home-built multi-sample dissolution dynamic nuclear polarization system. Phantom dissolution experiments were conducted to determine optimal parameters of the decarboxylation reaction, retaining polarization and T1 of [1-13 C]acetate. In vivo feasibility of detecting [1-13 C]acetate metabolism is demonstrated using slice-selective spectroscopy and multi-echo imaging of [1-13 C]acetate and [1-13 C]acetylcarnitine in the healthy rat heart.
RESULTS
The first in vivo signal was observed ~23 s after dissolution. At the corresponding time point in the phantom experiments, 97.9 ± 0.4% of [2-13 C]pyruvate were converted into [1-13 C]acetate by the decarboxylation reaction. T1 and polarization of [1-13 C]acetate was determined to be 29.7 ± 1.9% and a 47.7 ± 0.5 s. Polarization levels of [2-13 C]pyruvate and [1-13 C]acetate were not significantly different after transfer to the scanner. In vivo, [1-13 C]acetate and [1-13 C]acetylcarnitine could be detected using spectroscopy and imaging.
CONCLUSION
Decarboxylation of hyperpolarized [2-13 C]pyruvate enables the efficient production of highly polarized [1-13 C]acetate that is applicable to study short-chain fatty acid metabolism in the in vivo heart.
The objective of the present work was to develop and implement an efficient approach to hyperpolarize [1-13 C]acetate and apply it to in vivo cardiac spectroscopy and imaging.
METHODS
Rapid hydrogen peroxide induced decarboxylation was used to convert hyperpolarized [2-13 C]pyruvate into highly polarized [1-13 C]acetate employing an additional step following rapid dissolution of [2-13 C]pyruvate in a home-built multi-sample dissolution dynamic nuclear polarization system. Phantom dissolution experiments were conducted to determine optimal parameters of the decarboxylation reaction, retaining polarization and T1 of [1-13 C]acetate. In vivo feasibility of detecting [1-13 C]acetate metabolism is demonstrated using slice-selective spectroscopy and multi-echo imaging of [1-13 C]acetate and [1-13 C]acetylcarnitine in the healthy rat heart.
RESULTS
The first in vivo signal was observed ~23 s after dissolution. At the corresponding time point in the phantom experiments, 97.9 ± 0.4% of [2-13 C]pyruvate were converted into [1-13 C]acetate by the decarboxylation reaction. T1 and polarization of [1-13 C]acetate was determined to be 29.7 ± 1.9% and a 47.7 ± 0.5 s. Polarization levels of [2-13 C]pyruvate and [1-13 C]acetate were not significantly different after transfer to the scanner. In vivo, [1-13 C]acetate and [1-13 C]acetylcarnitine could be detected using spectroscopy and imaging.
CONCLUSION
Decarboxylation of hyperpolarized [2-13 C]pyruvate enables the efficient production of highly polarized [1-13 C]acetate that is applicable to study short-chain fatty acid metabolism in the in vivo heart.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.1002/mrm.27782
- PMID 31045272
- Persistent URL
- https://sonar.ch/global/documents/113202
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