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Hydrophobic interaction chromatography for the characterization of monoclonal antibodies and related products.

  • Fekete S School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Boulevard d'Yvoy 20, 1211 Geneva 4, Switzerland. Electronic address: szabolcs.fekete@unige.ch.
  • Veuthey JL School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Boulevard d'Yvoy 20, 1211 Geneva 4, Switzerland.
  • Beck A Center of Immunology Pierre Fabre, 5 Avenue Napoléon III, BP 60497, 74160 Saint-Julien-en-Genevois, France(1).
  • Guillarme D School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Boulevard d'Yvoy 20, 1211 Geneva 4, Switzerland.
  • 2016-04-17
Published in:
  • Journal of pharmaceutical and biomedical analysis. - 2016
Antibody-drug-conjugate
Columns
Hydrophobic interaction chromatography
Method development
Therapeutic antibody
Antibodies, Monoclonal
Chromatography, High Pressure Liquid
Chromatography, Reverse-Phase
Hydrophobic and Hydrophilic Interactions
English Hydrophobic interaction chromatography (HIC) is a historical strategy used for the analytical purification and characterization of proteins. Similarly to what can be done in reversed-phase liquid chromatography (RPLC), HIC is able to separate protein species based on their hydrophobicity, but using different conditions. Compared to RPLC, the main benefit of HIC is its ability to perform separations under non denaturing conditions (i.e. physiological pH conditions, ambient mobile phase temperature and no need for organic solvents) and so an orthogonal method. The goal of this review is to provide a general overview of theoretical and practical aspects of modern HIC applied for the characterization of therapeutic protein biopharmaceuticals including monoclonal antibodies (mAbs), antibody drug conjugates (ADCs) and bispecific antibodies (bsAbs). Therefore, method development approaches, state-of-the-art column technology, applications and future perspectives are described and critically discussed.
Language
  • English
Open access status
bronze
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Persistent URL
https://sonar.ch/global/documents/116292
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