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Rice tungro bacilliform virus: transcription and translation in protoplasts.
Journal article

Rice tungro bacilliform virus: transcription and translation in protoplasts.

  • 1994-10-01
Published in:
  • Virology. - 1994
Base Sequence
Gene Expression Regulation, Viral
Genome, Viral
Molecular Sequence Data
Nucleic Acid Conformation
Open Reading Frames
Plant Viruses
Plants
Promoter Regions, Genetic
Protein Biosynthesis
Protoplasts
RNA, Messenger
RNA, Viral
Recombinant Fusion Proteins
Sequence Deletion
Transcription, Genetic
English Protoplasts from cell suspension cultures of Oryza sativa (monocot) and Orychophragmus violaceus (dicot) support transcription from the rice tungro bacilliform virus (RTBV) promoter and translation of the resulting mRNA despite the presence of a long leader sequence with strong secondary structure and 12 short open reading frames. Transcriptional elements located both upstream and downstream of the transcription initiation site are defined by deletion analysis and the functional TATA motif is determined. Expression of an open reading frame downstream of the entire leader is more efficient than that downstream of truncated derivatives. For optimal expression sequences in the 5' and 3' parts of the leader are required.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/12012
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