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Efficient transformation of Lactococcus lactis IL1403 and generation of knock-out mutants by homologous recombination.
Journal article

Efficient transformation of Lactococcus lactis IL1403 and generation of knock-out mutants by homologous recombination.

  • Gerber SD Department of Clinical Pharmacology, University of Berne, Berne, Switzerland.
  • Solioz M
  • 2007-05-24
Published in:
  • Journal of basic microbiology. - 2007
Electroporation
Genetic Vectors
Lactococcus lactis
Mutation
Transformation, Bacterial
English Lactococcus lactis IL1403 is a Gram-positive bacterium of great biotechnological interest for food grade applications. Its use is however hampered by the difficulty to efficiently transform this strain. We here describe a detailed, optimized electrotransformation protocol which yields a transformation efficiency of 10(6) cfu/microg of DNA with the two E. coli Gram-positive shuttle vectors pC3 and pVA838. The utility of the protocol was demonstrated by the generation of single- and double-knock-out mutants by homologous recombination.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/1205
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