Increased lipopolysaccharide-induced tumour necrosis factor-alpha, interferon-gamma and interleukin-10 production in atopic dermatitis.

Simon D; Braathen LR; Simon HU

doi:10.1111/j.1365-2133.2007.08050.x

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Journal article

Increased lipopolysaccharide-induced tumour necrosis factor-alpha, interferon-gamma and interleukin-10 production in atopic dermatitis.

Simon D Department of Dermatology, Inselspital, University of Bern, CH-3010 Bern, Switzerland. dagmar.simon@insel.ch
Braathen LR
Simon HU

2007-06-29

Published in:

The British journal of dermatology. - 2007

Granulocyte-Macrophage Colony-Stimulating Factor

Interleukin 1 Receptor Antagonist Protein

English BACKGROUND
Atopic dermatitis (AD) is based on a genetic predisposition, but environmental factors may trigger skin inflammation. According to the hygiene hypothesis, decreased exposure to microbial products in early childhood does not allow sufficient maturation of the immune system that is associated with an increased risk of atopic sensitization.

OBJECTIVES
The effect of lipopolysaccharide (LPS) on the cytokine production of peripheral blood mononuclear cells (PBMC) of AD patients and nonatopic controls was studied.

PATIENTS AND METHODS
PBMC were isolated from heparinized blood of 10 patients with AD and 10 nonatopic individuals, suspended in culture medium and stimulated with LPS. Cytokine levels in the supernatants were measured by immunoassays. Results Upon stimulation with LPS, PBMC from AD patients produced significantly higher amounts of tumour necrosis factor-alpha, interferon-gamma and interleukin (IL)-10 compared with control PBMC. LPS stimulation blocked the increased spontaneous production of IL-4 and IL-5 by PBMC from AD patients, but had no effect on IL-13 production.

CONCLUSIONS
These results demonstrate that the effects of LPS stimulation depend on both the type of cytokine and the origin of PBMC. Endotoxin exposure is suggested to modulate the disease course of AD.

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