Journal article
Transient cell-specific EXO70A1 activity in the CASP domain and Casparian strip localization.
- Kalmbach L Department of Plant Molecular Biology, University of Lausanne, Biophore Building, UNIL-Sorge, 1015 Lausanne, Switzerland.
- Hématy K Department of Plant Molecular Biology, University of Lausanne, Biophore Building, UNIL-Sorge, 1015 Lausanne, Switzerland.
- De Bellis D Department of Plant Molecular Biology, University of Lausanne, Biophore Building, UNIL-Sorge, 1015 Lausanne, Switzerland.
- Barberon M Department of Plant Molecular Biology, University of Lausanne, Biophore Building, UNIL-Sorge, 1015 Lausanne, Switzerland.
- Fujita S Department of Plant Molecular Biology, University of Lausanne, Biophore Building, UNIL-Sorge, 1015 Lausanne, Switzerland.
- Ursache R Department of Plant Molecular Biology, University of Lausanne, Biophore Building, UNIL-Sorge, 1015 Lausanne, Switzerland.
- Daraspe J Electron Microscopy Facility, University of Lausanne, Biophore Building, UNIL-Sorge, 1015 Lausanne, Switzerland.
- Geldner N Department of Plant Molecular Biology, University of Lausanne, Biophore Building, UNIL-Sorge, 1015 Lausanne, Switzerland.
- 2017-04-25
Published in:
- Nature plants. - 2017
English
In a striking case of evolutionary convergence, polarized cell layers with ring-like diffusion barriers have evolved in both plant and animal lineages independently. In plants, ring-like Casparian strips become localized by the CASPARIAN STRIP MEMBRANE DOMAIN PROTEINS (CASPs). The mechanism of this striking localization, however, has remained enigmatic. Here we present a genetic screen aimed at isolating determinants of CASP localization. One of the mutants, lord of the rings 2 (lotr2)/exo70a1, displays dramatic de-localization of CASPs into randomly localized microdomains. EXO70A1 is a subunit of the exocyst complex, a central component of secretion in eukaryotes. Irradiation of EXO70 subunit genes in plants has suggested specialization of this conserved complex. Intriguingly, lotr2/exo70a1 does neither affect secretion of the CASPs, nor that of other membrane proteins in the endodermis, thus separating exocyst activity in localization from a general defect in secretion. Our results establish EXO70A1 as a central player in Casparian strip formation, generating a transient positional information that will be translated into a precisely localized cell wall modification.
- Language
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- English
- Open access status
- closed
- Identifiers
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- DOI 10.1038/nplants.2017.58
- PMID 28436943
- Persistent URL
- https://sonar.ch/global/documents/123263
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