Glycosylation as means of reducing sample complexity to enable quantitative proteomics.
- Domon B Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland. domon@imsb.biol.ethz.ch
- 2009-03-03
Published in:
- Proteomics. - 2009
English
Quantitative proteomics is a rapidly expanding field, in particular, the application to clinical biomarker studies for diagnosis or prognosis of diseases, and the systematic analysis of protein functions in biological systems. Isolation of a class of peptides or a subproteome enables reduction of sample complexity, which is essential to perform sensitive, quantitative analyses over a wider dynamic range of protein concentrations. Glycosylation is one of the most frequent PTMs, and glycans have unique chemical properties that can be leveraged to selectively enrich for a subset of peptides, and thus facilitate the downstream analysis. The isolation of glycopeptides and its benefits for mass spectrometric measurements is discussed.
- Language
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- English
- Open access status
- bronze
- Identifiers
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- DOI 10.1002/pmic.200800545
- PMID 19253302
- Persistent URL
- https://sonar.ch/global/documents/123693
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