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Tenascin-W is a specific marker of glioma-associated blood vessels and stimulates angiogenesis in vitro.
Journal article

Tenascin-W is a specific marker of glioma-associated blood vessels and stimulates angiogenesis in vitro.

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  • 2009-11-04
Published in:
  • FASEB journal : official publication of the Federation of American Societies for Experimental Biology. - 2010
Biomarkers, Tumor
Blood Vessels
Blotting, Western
Cell Enlargement
Cell Line
Cell Movement
Cells, Cultured
Endothelial Cells
Fluorescent Antibody Technique
Glioma
Humans
In Vitro Techniques
Neovascularization, Physiologic
Spheroids, Cellular
Tenascin
Umbilical Veins
English The microenvironment hosting a tumor actively participates in regulating tumor cell proliferation, migration, and invasion. Among the extracellular matrix proteins enriched in the stroma of carcinomas are the tenascin family members tenascin-C and tenascin-W. Whereas tenascin-C overexpression in gliomas is known to correlate with poor prognosis, the status of tenascin-W in brain tumors has not been investigated so far. In the present study, we analyzed protein levels of tenascin-W in 38 human gliomas and found expression of tenascin-W in 80% of the tumor samples, whereas no tenascin-W could be detected in control, nontumoral brain tissues. Double immunohistochemical staining of tenascin-W and von Willebrand factor revealed that tenascin-W is localized around blood vessels, exclusively in tumor samples. In vitro, the presence of tenascin-W increased the proportion of elongated human umbilical vein endothelial cells (HUVECs) and augmented the mean speed of cell migration. Furthermore, tenascin-W triggered sprouting of HUVEC spheroids to a similar extent as the proangiogenic factor tenascin-C. In conclusion, our study identifies tenascin-W as a candidate biomarker for brain tumor angiogenesis that could be used as a molecular target for therapy irrespective of the glioma subtype.-Martina, E., Degen, M., Rüegg, C., Merlo, A., Lino, M. M., Chiquet-Ehrismann, R., Brellier, F. Tenascin-W is a specific marker of glioma-associated blood vessels and stimulates angiogenesis in vitro.
Language
  • English
Open access status
green
Identifiers
Persistent URL
https://sonar.ch/global/documents/12541
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