Journal article
Visualization and interpretation of eukaryotic DNA replication intermediates in vivo by electron microscopy.
- Neelsen KJ Institute of Molecular Cancer Research, University of Zurich, Zurich, Switzerland.
- Chaudhuri AR
- Follonier C
- Herrador R
- Lopes M
- 2013-10-29
Published in:
- Methods in molecular biology (Clifton, N.J.). - 2014
Animals
Cell Extracts
Chromatin
Chromosomes, Artificial, Bacterial
Cross-Linking Reagents
DNA
DNA Replication
DNA, Cruciform
Eukaryotic Cells
Ficusin
Genome, Fungal
Male
Mammals
Microscopy, Electron
Nucleic Acid Denaturation
Saccharomyces cerevisiae
Spermatozoa
Xenopus
English
The detailed understanding of the DNA replication process requires structural insight. The combination of psoralen cross-linking and electron microscopy has been extensively exploited to reveal the fine architecture of in vivo DNA replication intermediates. This approach proved instrumental to uncover the basic mechanisms of DNA duplication, as well as the perturbation of this process by various forms of replication stress. The replication structures are stabilized in vivo (by psoralen cross-linking) prior to extraction and enrichment procedures, allowing their visualization at the transmission electron microscope. This chapter outlines the procedures required to visualize and interpret in vivo replication intermediates of genomic DNA, extracted from budding yeast, Xenopus egg extracts, or cultured mammalian cells.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.1007/978-1-62703-706-8_15
- PMID 24162989
- Persistent URL
- https://sonar.ch/global/documents/12635
Statistics
Document views: 41
File downloads: