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Inflammation boosts bacteriophage transfer between Salmonella spp.
Journal article

Inflammation boosts bacteriophage transfer between Salmonella spp.

  • Diard M Institute of Microbiology, ETH Zurich, Switzerland. mederic.diard@micro.biol.ethz.ch wolf-dietrich.hardt@micro.biol.ethz.ch.
  • Bakkeren E Institute of Microbiology, ETH Zurich, Switzerland.
  • Cornuault JK Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy en Josas, France.
  • Moor K Institute of Microbiology, ETH Zurich, Switzerland.
  • Hausmann A Institute of Microbiology, ETH Zurich, Switzerland.
  • Sellin ME Institute of Microbiology, ETH Zurich, Switzerland.
  • Loverdo C Laboratoire Jean Perrin (UMR 8237), CNRS-UPMC, 75005 Paris, France.
  • Aertsen A Department of Microbial and Molecular Systems, KU Leuven, Belgium.
  • Ackermann M Department of Environmental Systems Science, ETH Zurich, and Department of Environmental Microbiology, Eawag, Switzerland.
  • De Paepe M Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy en Josas, France.
  • Slack E Institute of Microbiology, ETH Zurich, Switzerland.
  • Hardt WD Institute of Microbiology, ETH Zurich, Switzerland. mederic.diard@micro.biol.ethz.ch wolf-dietrich.hardt@micro.biol.ethz.ch.
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  • 2017-03-18
Published in:
  • Science (New York, N.Y.). - 2017
Animals
Diarrhea
Disease Models, Animal
Enteritis
Inflammation
Intestines
Lysogeny
Mice
Mice, Inbred C57BL
SOS Response, Genetics
Salmonella Phages
Salmonella typhimurium
Vaccination
Viral Proteins
English Bacteriophage transfer (lysogenic conversion) promotes bacterial virulence evolution. There is limited understanding of the factors that determine lysogenic conversion dynamics within infected hosts. A murine Salmonella Typhimurium (STm) diarrhea model was used to study the transfer of SopEΦ, a prophage from STm SL1344, to STm ATCC14028S. Gut inflammation and enteric disease triggered >55% lysogenic conversion of ATCC14028S within 3 days. Without inflammation, SopEΦ transfer was reduced by up to 105-fold. This was because inflammation (e.g., reactive oxygen species, reactive nitrogen species, hypochlorite) triggers the bacterial SOS response, boosts expression of the phage antirepressor Tum, and thereby promotes free phage production and subsequent transfer. Mucosal vaccination prevented a dense intestinal STm population from inducing inflammation and consequently abolished SopEΦ transfer. Vaccination may be a general strategy for blocking pathogen evolution that requires disease-driven transfer of temperate bacteriophages.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/127191
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