Journal article
Polycations as displacer in high-performance bioseparation.
- Schmidt B Institute of Chemical Engineering, Swiss Federal Institute of Technology Lausanne, Ecublens, Switzerland.
- Wandrey C
- Vogt S
- Freitag R
- Holzapfel H
- 2000-02-16
Published in:
- Journal of chromatography. A. - 1999
Chromatography, Ion Exchange
Cytochrome c Group
Molecular Weight
Muramidase
Polyamines
Polyethylenes
Quaternary Ammonium Compounds
English
Displacement chromatography is an interesting but up to now rarely used type of preparative biochromatography. The lack of well-engineered and accessible displacer contributes to this phenomenon. In this paper a novel type of displacer is introduced for cation-exchange displacement chromatography, which will soon become commercially available. The molecule is a well-defined PolyDADMAC [poly(diallyldimethylammonium chloride)] with a molar mass of less than 35000 g/mol, an exclusively linear structure and a molar mass polydispersity of less than 1.5. A method for synthesizing such a polymer at high yields is described. The PolyDADMAC is shown to be an efficient displacer of basic proteins from strong cation-exchange columns.
- Language
-
- English
- Open access status
- closed
- Identifiers
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- DOI 10.1016/s0021-9673(99)00809-2
- PMID 10674928
- Persistent URL
- https://sonar.ch/global/documents/127299
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