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Anaerobic Production of Poly(3-hydroxybutyrate) and Its Precursor 3-Hydroxybutyrate from Synthesis Gas by Autotrophic Clostridia.
Journal article

Anaerobic Production of Poly(3-hydroxybutyrate) and Its Precursor 3-Hydroxybutyrate from Synthesis Gas by Autotrophic Clostridia.

  • Flüchter S Institut für Mikrobiologie und Biotechnologie, Universität Ulm , Albert-Einstein-Allee 11 , 89081 Ulm , Germany.
  • Follonier S Institute of Life Technologies, University of Applied Sciences and Arts Western Switzerland (HES-SO Valais) , Route du Rawyl 64 , 1950 Sion , Switzerland.
  • Schiel-Bengelsdorf B Institut für Mikrobiologie und Biotechnologie, Universität Ulm , Albert-Einstein-Allee 11 , 89081 Ulm , Germany.
  • Bengelsdorf FR Institut für Mikrobiologie und Biotechnologie, Universität Ulm , Albert-Einstein-Allee 11 , 89081 Ulm , Germany.
  • Zinn M Institute of Life Technologies, University of Applied Sciences and Arts Western Switzerland (HES-SO Valais) , Route du Rawyl 64 , 1950 Sion , Switzerland.
  • Dürre P Institut für Mikrobiologie und Biotechnologie, Universität Ulm , Albert-Einstein-Allee 11 , 89081 Ulm , Germany.
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  • 2019-05-09
Published in:
  • Biomacromolecules. - 2019
3-Hydroxybutyric Acid
Autotrophic Processes
Bacteria, Anaerobic
Clostridium
Gases
Hydroxybutyrates
Polyesters
English Anaerobic production of the biopolymer poly(3-hydroxybutyrate) (PHB) and the monomer 3-hydroxybutyrate (3-HB) was achieved using recombinant clostridial acetogens supplied with syn(thesis) gas as the sole carbon and energy source. 3-HB production was successfully accomplished by a new synthetic pathway containing the genes thlA (encoding thiolase A), ctfA/B (encoding CoA-transferase A/B), and bdhA (encoding (R)-3-hydroxybutyrate dehydrogenase). The respective recombinant Clostridium coskatii [p83_tcb] strain produced autotrophically 0.98 ± 0.12 mM and heterotrophically 21.7 ± 0.27 mM 3-HB. As a proof of concept, production of PHB was achieved using recombinant C. coskatii and Clostridium ljungdahlii strains expressing a novel synthetic PHB pathway containing the genes thlA (encoding thiolase A), hbd (encoding 3-hydroxybutyryl-CoA dehydrogenase), crt (encoding crotonase), phaJ (encoding (R)-enoyl-CoA hydratase), and phaEC (encoding PHA synthase). The strain C. coskatii [p83_PHB_Scaceti] synthesized heterotrophically 3.4 ± 0.29% PHB per cell dry weight (CDW) and autotrophically 1.12 ± 0.12% PHB per CDW.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/128977
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