Journal article
Methylated DNA immunoprecipitation (MeDIP).
- Mohn F Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.
- Weber M
- Schübeler D
- Roloff TC
- 2008-11-07
Published in:
- Methods in molecular biology (Clifton, N.J.). - 2009
Animals
Base Sequence
CpG Islands
DNA
DNA Methylation
DNA Primers
Humans
Immunoprecipitation
Oligonucleotide Array Sequence Analysis
Viral Proteins
English
Methylated DNA immunoprecipitation (MeDIP) is a versatile immunocapturing approach for unbiased detection of methylated DNA. In brief, genomic DNA is randomly sheared by sonication and immunoprecipitated with a monoclonal antibody that specifically recognizes 5-methylcytidine. The resulting enrichment of methylated DNA in the immunoprecipitated fraction can be determined by PCR to assess the methylation state of individual regions. Alternatively, MeDIP can be combined with large-scale analysis using microarrays as a genome-wide experimental readout. This protocol has been applied to generate comprehensive DNA methylation profiles on a genome-wide scale in mammals and plants, and further to identify abnormally methylated genes in cancer cells.
- Language
-
- English
- Open access status
- closed
- Identifiers
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- DOI 10.1007/978-1-59745-522-0_5
- PMID 18987806
- Persistent URL
- https://sonar.ch/global/documents/129716
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