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Genomic organisation and expression of mouse deoxyribonuclease I.
Journal article

Genomic organisation and expression of mouse deoxyribonuclease I.

  • 1995-02-06
Published in:
  • Biochemical and biophysical research communications. - 1995
Amino Acid Sequence
Animals
Base Sequence
Cattle
DNA Primers
DNA, Complementary
Deoxyribonuclease I
Exons
Gene Expression
Gene Library
Humans
Mice
Molecular Sequence Data
Myocardium
Polymerase Chain Reaction
Protein Structure, Secondary
Rats
Restriction Mapping
Sequence Homology, Amino Acid
Sheep
Swine
English Deoxyribonuclease I (DNase I) has recently been implicated in cell death by apoptosis, a process which is frequently accompanied by chromatin DNA degradation. Despite extensive studies on DNase I, its genomic organisation remained unknown. Here we report for the first time on the intron-exon structure of the DNase I gene. The coding region of mouse DNase I is composed of eight introns and eight exons, spanning 2315 base pairs. The deduced protein sequence is 91.5% identical to its rat counterpart, but does not carry the two mutations (Glu13 to D and V67 to I) responsible for the decrease in actin-binding of rat DNase I. The enzymatic activity of mouse DNase I is found in striated muscle, kidney, intestine, liver, lymphnodes, but not in the heart, spleen or pancreas.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/130580
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