On the substrate- and stereospecificity of the plant carotenoid cleavage dioxygenase 7.
- Bruno M Albert-Ludwigs University of Freiburg, Faculty of Biology, Schaenzlestr. 1, D-79104 Freiburg, Germany.
- Hofmann M Albert-Ludwigs University of Freiburg, Faculty of Biology, Schaenzlestr. 1, D-79104 Freiburg, Germany.
- Vermathen M Department of Chemistry and Biochemistry, University of Bern, 3012 Bern, Switzerland.
- Alder A Albert-Ludwigs University of Freiburg, Faculty of Biology, Schaenzlestr. 1, D-79104 Freiburg, Germany.
- Beyer P Albert-Ludwigs University of Freiburg, Faculty of Biology, Schaenzlestr. 1, D-79104 Freiburg, Germany.
- Al-Babili S Albert-Ludwigs University of Freiburg, Faculty of Biology, Schaenzlestr. 1, D-79104 Freiburg, Germany; Center for Desert Agriculture, BESE Division, King Abdullah University of Science and Technology (KAUST), 23955-6900 Thuwal, Saudi Arabia. Electronic address: salim.babili@kaust.edu.sa.
- 2014-04-02
Published in:
- FEBS letters. - 2014
Apocarotenoid
Carlactone
Carotenoid
Carotenoid cleavage dioxygenase
Strigolactone
Arabidopsis
Arabidopsis Proteins
Carotenoids
Dioxygenases
Kinetics
Magnetic Resonance Spectroscopy
Peas
Stereoisomerism
Substrate Specificity
English
Strigolactones are phytohormones synthesized from carotenoids via a stereospecific pathway involving the carotenoid cleavage dioxygenases 7 (CCD7) and 8. CCD7 cleaves 9-cis-β-carotene to form a supposedly 9-cis-configured β-apo-10'-carotenal. CCD8 converts this intermediate through a combination of yet undetermined reactions into the strigolactone-like compound carlactone. Here, we investigated the substrate and stereo-specificity of the Arabidopsis and pea CCD7 and determined the stereo-configuration of the β-apo-10'-carotenal intermediate by using Nuclear Magnetic Resonance Spectroscopy. Our data unequivocally demonstrate the 9-cis-configuration of the intermediate. Both CCD7s cleave different 9-cis-carotenoids, yielding hydroxylated 9-cis-apo-10'-carotenals that may lead to hydroxylated carlactones, but show highest affinity for 9-cis-β-carotene.
- Language
-
- English
- Open access status
- bronze
- Identifiers
-
- DOI 10.1016/j.febslet.2014.03.041
- PMID 24685691
- Persistent URL
- https://sonar.ch/global/documents/131843
Statistics
Document views: 74
File downloads:
- Full-text: 0