Comparison of Serum Spec fPL(™) and 1,2-o-Dilauryl-Rac-Glycero-3-Glutaric Acid-(6'-Methylresorufin) Ester Assay in 60 Cats Using Standardized Assessment of Pancreatic Histology.
- Oppliger S Clinic for Small Animal Internal Medicine, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
- Hilbe M Institute for Veterinary Pathology, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
- Hartnack S Section of Epidemiology, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
- Zini E Clinic for Small Animal Internal Medicine, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
- Reusch CE Clinic for Small Animal Internal Medicine, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
- Kook PH Clinic for Small Animal Internal Medicine, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
- 2016-03-13
Published in:
- Journal of veterinary internal medicine. - 2016
Feline
Feline pancreas-specific lipase
Histopathology
Lipase
Pancreas
Animals
Azotemia
Biological Assay
Cat Diseases
Cats
Female
Glutarates
Lipase
Male
Oxazines
Pancreas
Pancreatitis
Sensitivity and Specificity
English
BACKGROUND
Feline pancreas-specific lipase (Spec fPL) is considered a useful test for the antemortem diagnosis of pancreatitis in cats. A recent study found good agreement between the results of the Spec fPL and catalytic 1,2-o-dilauryl-rac-glycero-3-glutaric acid-(6'-methylresorufin) ester (DGGR) lipase assay. Prospective studies evaluating their sensitivity and specificity are lacking.
OBJECTIVES
To compare the results of the Spec fPL and the DGGR assays with a standardized histologic assessment of the pancreas.
ANIMALS
Sixty client-owned cats presented for necropsy.
METHODS
PROSPECTIVE STUDY
Spec fPL concentrations and serum DGGR lipase activity were measured from the same blood sample. The pancreas was removed within 3 hours after euthanasia; serial transverse sections were made every 0.5 cm throughout the entire pancreas and reviewed using a histologic grading scheme. Sensitivity and specificity for the Spec fPL and DGGR assay results were determined.
RESULTS
The sensitivity and specificity for the Spec fPL assay (cutoff value ≥5.4 μg/L) was 42.1 [95% confidence interval (95% CI), 29.4-55.9%] and 100% (95% CI, 31.0-100.0%). The sensitivity and specificity for the DGGR assay (cutoff value >26 U/L) was 36.8 (95% CI, 24.7-50.7%) and 100% (95% CI, 31.0-100.0%). When lymphocytic inflammation up to 10% of a section was considered normal, the sensitivity and specificity for Spec fPL assay (cutoff value ≥5.4 μg/L) was 61.1 (95% CI, 36.1-81.7%) and 69.0% (95% CI, 52.8-81.9%) and the sensitivity and specificity for the DGGR assay (cutoff value >26 U/L) was 66.7 (95% CI, 41.2-85.6%) and 78.6% (95% CI, 62.8-89.2%).
CONCLUSIONS AND CLINICAL IMPORTANCE
Both lipase assays performed similarly well, but their agreement with histologic pancreatic inflammation was limited.
Feline pancreas-specific lipase (Spec fPL) is considered a useful test for the antemortem diagnosis of pancreatitis in cats. A recent study found good agreement between the results of the Spec fPL and catalytic 1,2-o-dilauryl-rac-glycero-3-glutaric acid-(6'-methylresorufin) ester (DGGR) lipase assay. Prospective studies evaluating their sensitivity and specificity are lacking.
OBJECTIVES
To compare the results of the Spec fPL and the DGGR assays with a standardized histologic assessment of the pancreas.
ANIMALS
Sixty client-owned cats presented for necropsy.
METHODS
PROSPECTIVE STUDY
Spec fPL concentrations and serum DGGR lipase activity were measured from the same blood sample. The pancreas was removed within 3 hours after euthanasia; serial transverse sections were made every 0.5 cm throughout the entire pancreas and reviewed using a histologic grading scheme. Sensitivity and specificity for the Spec fPL and DGGR assay results were determined.
RESULTS
The sensitivity and specificity for the Spec fPL assay (cutoff value ≥5.4 μg/L) was 42.1 [95% confidence interval (95% CI), 29.4-55.9%] and 100% (95% CI, 31.0-100.0%). The sensitivity and specificity for the DGGR assay (cutoff value >26 U/L) was 36.8 (95% CI, 24.7-50.7%) and 100% (95% CI, 31.0-100.0%). When lymphocytic inflammation up to 10% of a section was considered normal, the sensitivity and specificity for Spec fPL assay (cutoff value ≥5.4 μg/L) was 61.1 (95% CI, 36.1-81.7%) and 69.0% (95% CI, 52.8-81.9%) and the sensitivity and specificity for the DGGR assay (cutoff value >26 U/L) was 66.7 (95% CI, 41.2-85.6%) and 78.6% (95% CI, 62.8-89.2%).
CONCLUSIONS AND CLINICAL IMPORTANCE
Both lipase assays performed similarly well, but their agreement with histologic pancreatic inflammation was limited.
- Language
-
- English
- Open access status
- gold
- Identifiers
-
- DOI 10.1111/jvim.13924
- PMID 26968865
- Persistent URL
- https://sonar.ch/global/documents/133129
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