Precision and accuracy of single-molecule FRET measurements-a multi-laboratory benchmark study.

• Hellenkamp B Institute of Physical Chemistry, University of Freiburg, Freiburg im Breisgau, Germany.
• Schmid S Institute of Physical Chemistry, University of Freiburg, Freiburg im Breisgau, Germany.
• Doroshenko O Molecular Physical Chemistry, Heinrich-Heine-Universität Düsseldorf, Düsseldorf, Germany.
• Opanasyuk O Molecular Physical Chemistry, Heinrich-Heine-Universität Düsseldorf, Düsseldorf, Germany.
• Kühnemuth R Molecular Physical Chemistry, Heinrich-Heine-Universität Düsseldorf, Düsseldorf, Germany.
• Rezaei Adariani S Department of Physics and Astronomy, Clemson University, Clemson, SC, USA.
• Ambrose B Department of Chemistry, University of Sheffield, Sheffield, UK.
• Aznauryan M Interdisciplinary Nanoscience Center (iNANO) and Department of Chemistry, Aarhus University, Aarhus, Denmark.
• Barth A Physical Chemistry, Department of Chemistry, Nanosystems Initiative Munich (NIM), Center for Integrated Protein Science Munich (CiPSM) and Center for Nanoscience (CeNS), Ludwig-Maximilians-Universität München, Munich, Germany.
• Birkedal V Interdisciplinary Nanoscience Center (iNANO) and Department of Chemistry, Aarhus University, Aarhus, Denmark.
• Bowen ME Department of Physiology & Biophysics, Stony Brook University, Stony Brook, NY, USA.
• Chen H Department of Biomedical Engineering, University of California, Irvine, Irvine, CA, USA.
• Cordes T Molecular Microscopy Research Group, Zernike Institute for Advanced Materials, University of Groningen, Groningen, the Netherlands.
• Eilert T Institute for Biophysics, Ulm University, Ulm, Germany.
• Fijen C Laboratory of Biophysics, Wageningen University & Research, Wageningen, the Netherlands.
• Gebhardt C Physical and Synthetic Biology, Faculty of Biology, Ludwig-Maximilians-Universität München, Planegg-Martinsried, Germany.
• Götz M Institute of Physical Chemistry, University of Freiburg, Freiburg im Breisgau, Germany.
• Gouridis G Molecular Microscopy Research Group, Zernike Institute for Advanced Materials, University of Groningen, Groningen, the Netherlands.
• Gratton E Department of Biomedical Engineering, University of California, Irvine, Irvine, CA, USA.
• Ha T Department of Biomedical Engineering, John Hopkins University, Baltimore, MD, USA.
• Hao P Department of Physics, North Carolina State University, Raleigh, NC, USA.
• Hanke CA Molecular Physical Chemistry, Heinrich-Heine-Universität Düsseldorf, Düsseldorf, Germany.
• Hartmann A B CUBE-Center for Molecular Bioengineering, TU Dresden, Dresden, Germany.
• Hendrix J Laboratory for Photochemistry and Spectroscopy, Department of Chemistry, University of Leuven, Leuven, Belgium.
• Hildebrandt LL Interdisciplinary Nanoscience Center (iNANO) and Department of Chemistry, Aarhus University, Aarhus, Denmark.
• Hirschfeld V Institute of Physics, University of Lübeck, Lübeck, Germany.
• Hohlbein J Laboratory of Biophysics, Wageningen University & Research, Wageningen, the Netherlands.
• Hua B Department of Biomedical Engineering, John Hopkins University, Baltimore, MD, USA.
• Hübner CG Institute of Physics, University of Lübeck, Lübeck, Germany.
• Kallis E Institute for Biophysics, Ulm University, Ulm, Germany.
• Kapanidis AN Gene Machines Group, Clarendon Laboratory, Department of Physics, University of Oxford, Oxford, UK.
• Kim JY School of Chemistry, Seoul National University, Seoul, South Korea.
• Krainer G B CUBE-Center for Molecular Bioengineering, TU Dresden, Dresden, Germany.
• Lamb DC Physical Chemistry, Department of Chemistry, Nanosystems Initiative Munich (NIM), Center for Integrated Protein Science Munich (CiPSM) and Center for Nanoscience (CeNS), Ludwig-Maximilians-Universität München, Munich, Germany.
• Lee NK School of Chemistry, Seoul National University, Seoul, South Korea.
• Lemke EA Departments of Biology and Chemistry, Pharmacy and Geosciences, Johannes Gutenberg-University Mainz, Mainz, Germany.
• Levesque B Department of Physiology & Biophysics, Stony Brook University, Stony Brook, NY, USA.
• Levitus M School of Molecular Sciences and The Biodesign Institute, Arizona State University, Tempe, AZ, USA.
• McCann JJ Department of Physiology & Biophysics, Stony Brook University, Stony Brook, NY, USA.
• Naredi-Rainer N Physical Chemistry, Department of Chemistry, Nanosystems Initiative Munich (NIM), Center for Integrated Protein Science Munich (CiPSM) and Center for Nanoscience (CeNS), Ludwig-Maximilians-Universität München, Munich, Germany.
• Nettels D Department of Biochemistry, University of Zurich, Zurich, Switzerland.
• Ngo T Department of Biomedical Engineering, John Hopkins University, Baltimore, MD, USA.
• Qiu R Department of Physics, North Carolina State University, Raleigh, NC, USA.
• Robb NC Gene Machines Group, Clarendon Laboratory, Department of Physics, University of Oxford, Oxford, UK.
• Röcker C Institute for Biophysics, Ulm University, Ulm, Germany.
• Sanabria H Department of Physics and Astronomy, Clemson University, Clemson, SC, USA.
• Schlierf M B CUBE-Center for Molecular Bioengineering, TU Dresden, Dresden, Germany.
• Schröder T Department of Chemistry, Ludwig-Maximilians-Universität München, München, Germany.
• Schuler B Department of Biochemistry, University of Zurich, Zurich, Switzerland.
• Seidel H Institute of Physics, University of Lübeck, Lübeck, Germany.
• Streit L Institute for Biophysics, Ulm University, Ulm, Germany.
• Thurn J Institute of Physical Chemistry, University of Freiburg, Freiburg im Breisgau, Germany.
• Tinnefeld P Department of Chemistry, Ludwig-Maximilians-Universität München, München, Germany.
• Tyagi S Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
• Vandenberk N Laboratory for Photochemistry and Spectroscopy, Department of Chemistry, University of Leuven, Leuven, Belgium.
• Vera AM Department of Chemistry, Ludwig-Maximilians-Universität München, München, Germany.
• Weninger KR Department of Physics, North Carolina State University, Raleigh, NC, USA.
• Wünsch B Institute of Physical & Theoretical Chemistry, Braunschweig Integrated Centre of Systems Biology (BRICS), and Laboratory for Emerging Nanometrology (LENA), Braunschweig University of Technology, Braunschweig, Germany.
• Yanez-Orozco IS Department of Physics and Astronomy, Clemson University, Clemson, SC, USA.
• Michaelis J Institute for Biophysics, Ulm University, Ulm, Germany. jens.michaelis@uni-ulm.de.
• Seidel CAM Molecular Physical Chemistry, Heinrich-Heine-Universität Düsseldorf, Düsseldorf, Germany. cseidel@hhu.de.
• Craggs TD Department of Chemistry, University of Sheffield, Sheffield, UK. t.craggs@sheffield.ac.uk.
• Hugel T Institute of Physical Chemistry, University of Freiburg, Freiburg im Breisgau, Germany. thorsten.hugel@pc.uni-freiburg.de.

Fluorescence Resonance Energy Transfer

Laboratories

Reproducibility of Results