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Labeling of Bifidobacterium longum Cells with 13C-Substituted Leucine for Quantitative Proteomic Analyses

  • Couté, Yohann Biomedical Proteomics Research Group, Department of Structural Biology and Bioinformatics, University of Geneva, 1 Rue Michel Servet, 1211 Geneva 14, Switzerland
  • Hernandez, Céline Proteome Informatics Group, Swiss Institute of Bioinformatics, Geneva, Switzerland
  • Appel, Ron D. Proteome Informatics Group, Swiss Institute of Bioinformatics, Geneva, Switzerland
  • Sanchez, Jean-Charles Biomedical Proteomics Research Group, Department of Structural Biology and Bioinformatics, University of Geneva, 1 Rue Michel Servet, 1211 Geneva 14, Switzerland
  • Margolles, Abelardo Instituto de Productos Lácteos de Asturias, Consejo Superior de Investigaciones Científicas (CSIC), Ctra. Infiesto s/n, 33300 Villaviciosa, Asturias, Spain
Published in:
  • Applied and Environmental Microbiology. - American Society for Microbiology. - 2007, vol. 73, no. 17, p. 5653-5656
Biotechnology
Food Science
Ecology
Applied Microbiology and Biotechnology
English ABSTRACT
Stable isotope labeling of amino acids in cell culture was used for Bifidobacterium longum. A comprehensive proteomic strategy was developed and validated by designing an appropriate semidefined medium that allows stable replacement of natural leucine by [13C6]leucine. Using this strategy, proteins having variations of at least 50% in their expression rates can be quantified with great confidence.
Language
  • English
Open access status
bronze
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https://sonar.ch/global/documents/1362
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