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Design-based stereology: Planning, volumetry and sampling are crucial steps for a successful study.
Journal article

Design-based stereology: Planning, volumetry and sampling are crucial steps for a successful study.

  • Tschanz S Institute of Anatomy, University of Bern, Bern, Switzerland.
  • Schneider JP Institute of Functional and Applied Anatomy, Hannover Medical School, Hannover, Germany.
  • Knudsen L Institute of Functional and Applied Anatomy, Hannover Medical School, Hannover, Germany. Electronic address: knudsen.lars@mh-hannover.de.
  • 2013-06-18
Published in:
  • Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft. - 2014
Cavalieri method
Design-based stereology
Fractionator
Systematic uniform random sample
Volumetry
Algorithms
Anatomy
Animals
Anisotropy
Cell Count
Humans
Image Processing, Computer-Assisted
Imaging, Three-Dimensional
Microscopy
Microscopy, Electron
Reproducibility of Results
Specimen Handling
English Quantitative data obtained by means of design-based stereology can add valuable information to studies performed on a diversity of organs, in particular when correlated to functional/physiological and biochemical data. Design-based stereology is based on a sound statistical background and can be used to generate accurate data which are in line with principles of good laboratory practice. In addition, by adjusting the study design an appropriate precision can be achieved to find relevant differences between groups. For the success of the stereological assessment detailed planning is necessary. In this review we focus on common pitfalls encountered during stereological assessment. An exemplary workflow is included, and based on authentic examples, we illustrate a number of sampling principles which can be implemented to obtain properly sampled tissue blocks for various purposes.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/1386
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