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Adsorption and Bioactivity of Protein A on Silicon Surfaces Studied by AFM and XPS.
Journal article

Adsorption and Bioactivity of Protein A on Silicon Surfaces Studied by AFM and XPS.

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  • 2000-12-21
Published in:
  • Journal of colloid and interface science. - 2001
Colloid and Surface Chemistry
Electronic, Optical and Magnetic Materials
Surfaces, Coatings and Films
Biomaterials
English The adsorption of protein A on silicon surfaces was studied by atomic force microscopy (AFM) and X-ray photoelectron spectroscopy. The deposition was made statically from various concentrations of protein A in water solution. The biological activity was checked by the immobilization of rabbit immunoglobulin G. The protein adsorption occurs in least two different phases and leads to a multilayer film. The first monolayer of proteins is rapidly adsorbed on the surface. The adsorption of the second layer of proteins occurs much more slowly (a thousand times slower) and also involves the third monolayer. The protein A of the first monolayer is denaturated and biologically inactive. On the contrary, the proteins of the second monolayer keep their natural diameter and remain biologically active. AFM artifacts such as the convolution with small objects and the resulting estimation of the coverage ratio are discussed. Copyright 2001 Academic Press.
Language
  • English
Open access status
closed
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Persistent URL
https://sonar.ch/global/documents/138724
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