Back
LET-418/Mi2 and SPR-5/LSD1 cooperatively prevent somatic reprogramming of C. elegans germline stem cells.
Journal article

LET-418/Mi2 and SPR-5/LSD1 cooperatively prevent somatic reprogramming of C. elegans germline stem cells.

  • Käser-Pébernard S Department of Zoology, University of Fribourg, Chemin du musée 10, 1700 Fribourg, Switzerland.
  • Müller F Department of Zoology, University of Fribourg, Chemin du musée 10, 1700 Fribourg, Switzerland.
  • Wicky C Department of Zoology, University of Fribourg, Chemin du musée 10, 1700 Fribourg, Switzerland.
  • 2014-04-22
Published in:
  • Stem cell reports. - 2014
Animals
Caenorhabditis elegans
Caenorhabditis elegans Proteins
Cell Differentiation
Cell Division
Cell Transformation, Neoplastic
Cellular Reprogramming
Chromatin
DNA-Binding Proteins
Down-Regulation
Female
Gene Expression Regulation
Germ Cells
Histones
Male
Methylation
Mi-2 Nucleosome Remodeling and Deacetylase Complex
Mutation
Neoplasms, Germ Cell and Embryonal
Neurons
Oxidoreductases, N-Demethylating
Protein Binding
Stem Cells
English Throughout their journey to forming new individuals, germline stem cells must remain totipotent, particularly by maintaining a specific chromatin structure. However, the place epigenetic factors occupy in this process remains elusive. So far, "sensitization" of chromatin by modulation of histone arrangement and/or content was believed to facilitate transcription-factor-induced germ cell reprogramming. Here, we demonstrate that the combined reduction of two epigenetic factors suffices to reprogram C. elegans germ cells. The histone H3K4 demethylase SPR-5/LSD1 and the chromatin remodeler LET-418/Mi2 function together in an early process to maintain germ cell status and act as a barrier to block precocious differentiation. This epigenetic barrier is capable of limiting COMPASS-mediated H3K4 methylation, because elevated H3K4me3 levels correlate with germ cell reprogramming in spr-5; let-418 mutants. Interestingly, germ cells deficient for spr-5 and let-418 mainly reprogram as neurons, suggesting that neuronal fate might be the first to be derepressed in early embryogenesis.
Language
  • English
Open access status
gold
Identifiers
Persistent URL
https://sonar.ch/global/documents/138846
Statistics
Document views: 17 File downloads: