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Journal article

Extrafollicular plasmablast B cells play a key role in carrying retroviral infection to peripheral organs.

  • 2001-05-09
Published in:
  • Journal of immunology (Baltimore, Md. : 1950). - 2001
Animals
Antigens, CD
B-Lymphocyte Subsets
Cell Differentiation
Cell Movement
Female
Hindlimb
Integrin alpha4
Integrin alpha4beta1
Integrin beta1
Integrins
Lymph Nodes
Lymphocyte Activation
Lymphoid Tissue
Mammary Neoplasms, Experimental
Mammary Tumor Virus, Mouse
Mice
Mice, Inbred BALB C
Plasma Cells
Receptors, Lymphocyte Homing
Retroviridae Infections
Tumor Virus Infections
English B cells can either differentiate in germinal centers or in extrafollicular compartments of secondary lymphoid organs. Here we show the migration properties of B cells after differentiation in murine peripheral lymph node infected with mouse mammary tumor virus. Naive B cells become activated, infected, and carry integrated retroviral DNA sequences. After production of a retroviral superantigen, the infected B cells receive cognate T cell help and differentiate along the two main differentiation pathways analogous to classical Ag responses. The extrafollicular differentiation peaks on day 6 of mouse mammary tumor virus infection, and the follicular one becomes detectable after day 10. B cells participating in this immune response carry a retroviral DNA marker that can be detected by using semiquantitative PCR. We determined the migration patterns of B cells having taken part in the T cell-B cell interaction from the draining lymph node to different tissues. Waves of immigration and retention of infected cells in secondary lymphoid organs, mammary gland, salivary gland, skin, lung, and liver were observed correlating with the two peaks of B cell differentiation in the draining lymph node. Other organs revealed immigration of infected cells at later time points. The migration properties were correlated with a strong up-regulation of alpha(4)beta(1) integrin expression. These results show the migration properties of B cells during an immune response and demonstrate that a large proportion of extrafolliculary differentiating plasmablasts can escape local cell death and carry the retroviral infection to peripheral organs.
Language
  • English
Open access status
bronze
Identifiers
Persistent URL
https://sonar.ch/global/documents/139164
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