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Dynamics in protein translation sustaining T cell preparedness.
Journal article

Dynamics in protein translation sustaining T cell preparedness.

  • Wolf T Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland.
  • Jin W Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland.
  • Zoppi G Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland.
  • Vogel IA Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland.
  • Akhmedov M Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland.
  • Bleck CKE Biozentrum, University of Basel, Basel, Switzerland.
  • Beltraminelli T Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland.
  • Rieckmann JC Experimental Systems Immunology, Max Planck Institute of Biochemistry, Munich, Germany.
  • Ramirez NJ Institute for Immunodeficiency, Center for Chronic Immunodeficiency, Medical Center, Faculty of Medicine, Albert-Ludwigs-University of Freiburg, Freiburg, Germany.
  • Benevento M Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland.
  • Notarbartolo S Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland.
  • Bumann D Biozentrum, University of Basel, Basel, Switzerland.
  • Meissner F Experimental Systems Immunology, Max Planck Institute of Biochemistry, Munich, Germany.
  • Grimbacher B Institute for Immunodeficiency, Center for Chronic Immunodeficiency, Medical Center, Faculty of Medicine, Albert-Ludwigs-University of Freiburg, Freiburg, Germany.
  • Mann M Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Munich, Germany.
  • Lanzavecchia A Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland.
  • Sallusto F Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland.
  • Kwee I Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland.
  • Geiger R Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland. roger.geiger@irb.usi.ch.
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  • 2020-07-08
Published in:
  • Nature immunology. - 2020
Humans
Lymphocyte Activation
Protein Biosynthesis
RNA, Messenger
T-Lymphocytes
Transcription Factors
English In response to pathogenic threats, naive T cells rapidly transition from a quiescent to an activated state, yet the underlying mechanisms are incompletely understood. Using a pulsed SILAC approach, we investigated the dynamics of mRNA translation kinetics and protein turnover in human naive and activated T cells. Our datasets uncovered that transcription factors maintaining T cell quiescence had constitutively high turnover, which facilitated their depletion following activation. Furthermore, naive T cells maintained a surprisingly large number of idling ribosomes as well as 242 repressed mRNA species and a reservoir of glycolytic enzymes. These components were rapidly engaged following stimulation, promoting an immediate translational and glycolytic switch to ramp up the T cell activation program. Our data elucidate new insights into how T cells maintain a prepared state to mount a rapid immune response, and provide a resource of protein turnover, absolute translation kinetics and protein synthesis rates in T cells ( https://www.immunomics.ch ).
Language
  • English
Open access status
closed
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Persistent URL
https://sonar.ch/global/documents/139283
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