Journal article
LC-MS/MS method for nine different antibiotics.
- Rehm S Laboratory Medicine, University Hospital Basel, University Basel, Petersgraben 4, 4031 Basel, Switzerland.
- Rentsch KM Laboratory Medicine, University Hospital Basel, University Basel, Petersgraben 4, 4031 Basel, Switzerland. Electronic address: katharina.rentsch@usb.ch.
- 2020-11-07
Published in:
- Clinica chimica acta; international journal of clinical chemistry. - 2020
Antibiotics
High pressure liquid chromatography
Stability
Tandem mass spectrometry
Therapeutic drug monitoring
English
BACKGROUND AND AIMS
TDM of antibiotics can bring benefits to patients and healthcare systems by providing better treatment and saving healthcare resources. We aimed to develop a multi-analyte method for several diverse antibiotics using LC-MS/MS.
MATERIALS AND METHODS
Sample preparation consisted of protein precipitation with methanol, dilution and online extraction using a Turboflow Cyclone column. Separation was performed on a Synergi 4 µm Max RP column and deuterated forms of three antibiotics were used as internal standards.
RESULTS
We present a LC-MS/MS method for the quantitative determination of nine antibiotics, including five cephalosporins, the carbapenem ertapenem, the fluoroquinolone ciprofloxacin as well as the combination drug trimethoprim-sulfamethoxazole from plasma. Additionally, unbound ertapenem and cefazolin were analyzed in plasma water after ultrafiltration using plasma calibrators. Results from routine TDM show the applicability of the method.
CONCLUSION
The presented method is precise and accurate and was introduced in a university hospital, permitting fast TDM of all nine analytes. It was also used in a clinical study for measuring cefazolin free and total concentrations.
TDM of antibiotics can bring benefits to patients and healthcare systems by providing better treatment and saving healthcare resources. We aimed to develop a multi-analyte method for several diverse antibiotics using LC-MS/MS.
MATERIALS AND METHODS
Sample preparation consisted of protein precipitation with methanol, dilution and online extraction using a Turboflow Cyclone column. Separation was performed on a Synergi 4 µm Max RP column and deuterated forms of three antibiotics were used as internal standards.
RESULTS
We present a LC-MS/MS method for the quantitative determination of nine antibiotics, including five cephalosporins, the carbapenem ertapenem, the fluoroquinolone ciprofloxacin as well as the combination drug trimethoprim-sulfamethoxazole from plasma. Additionally, unbound ertapenem and cefazolin were analyzed in plasma water after ultrafiltration using plasma calibrators. Results from routine TDM show the applicability of the method.
CONCLUSION
The presented method is precise and accurate and was introduced in a university hospital, permitting fast TDM of all nine analytes. It was also used in a clinical study for measuring cefazolin free and total concentrations.
- Language
-
- English
- Open access status
- hybrid
- Identifiers
-
- DOI 10.1016/j.cca.2020.11.001
- PMID 33159947
- Persistent URL
- https://sonar.ch/global/documents/139350
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