Bioinformatic and Comparative Localization of Rab Proteins Reveals Functional Insights into the Uncharacterized GTPases Ypt10p and Ypt11p
- Buvelot Frei, Stéphanie Institut für Biochemie, Swiss Federal Institute of Technology (ETH), CH-8093 Zürich, Switzerland
- Rahl, Peter B. Graduate Program in Pharmacology, Cornell University, Ithaca, New York 14853
- Nussbaum, Maria Research Apprenticeship in Biological Sciences, Cornell University Summer College, Ithaca, New York 14853
- Briggs, Benjamin J. Honors Program in Undergraduate Biology, Cornell University, Ithaca, New York 14853
- Calero, Monica Graduate Program in Pharmacology, Cornell University, Ithaca, New York 14853
- Janeczko, Stephanie Leadership Program for Veterinary Students, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853
- Regan, Andrew D. Graduate Program in Microbiology, Cornell University, Ithaca, New York 14853
- Chen, Catherine Z. Graduate Program in Pharmacology, Cornell University, Ithaca, New York 14853
- Barral, Yves Institut für Biochemie, Swiss Federal Institute of Technology (ETH), CH-8093 Zürich, Switzerland
- Whittaker, Gary R. Microbiology and Immunology, Cornell University, Ithaca, New York 14853
- Collins, Ruth N. Departments of Molecular Medicine
Published in:
- Molecular and Cellular Biology. - American Society for Microbiology. - 2006, vol. 26, no. 19, p. 7299-7317
English
ABSTRACT
A
striking characteristic of a Rab protein is its steady-state
localization to the cytosolic surface of a particular subcellular
membrane. In this study, we have undertaken a combined bioinformatic
and experimental approach to examine the evolutionary conservation of
Rab protein localization. A comprehensive primary sequence
classification shows that 10 out of the 11 Rab proteins identified in
the yeast (Saccharomyces cerevisiae ) genome can be
grouped within a major subclass, each comprising multiple Rab orthologs
from diverse species. We compared the locations of individual yeast Rab
proteins with their localizations following ectopic expression in
mammalian cells. Our results suggest that green fluorescent
protein-tagged Rab proteins maintain localizations across large
evolutionary distances and that the major known player in the Rab
localization pathway, mammalian Rab-GDI, is able to function in yeast.
These findings enable us to provide insight into novel gene functions
and classify the uncharacterized Rab proteins Ypt10p (YBR264C) as being
involved in endocytic function and Ypt11p (YNL304W) as being localized
to the endoplasmic reticulum, where we demonstrate it is required for
organelle
inheritance.
striking characteristic of a Rab protein is its steady-state
localization to the cytosolic surface of a particular subcellular
membrane. In this study, we have undertaken a combined bioinformatic
and experimental approach to examine the evolutionary conservation of
Rab protein localization. A comprehensive primary sequence
classification shows that 10 out of the 11 Rab proteins identified in
the yeast (
grouped within a major subclass, each comprising multiple Rab orthologs
from diverse species. We compared the locations of individual yeast Rab
proteins with their localizations following ectopic expression in
mammalian cells. Our results suggest that green fluorescent
protein-tagged Rab proteins maintain localizations across large
evolutionary distances and that the major known player in the Rab
localization pathway, mammalian Rab-GDI, is able to function in yeast.
These findings enable us to provide insight into novel gene functions
and classify the uncharacterized Rab proteins Ypt10p (YBR264C) as being
involved in endocytic function and Ypt11p (YNL304W) as being localized
to the endoplasmic reticulum, where we demonstrate it is required for
organelle
inheritance.
- Language
-
- English
- Open access status
- bronze
- Identifiers
-
- DOI 10.1128/mcb.02405-05
- ISSN 0270-7306
- Persistent URL
- https://sonar.ch/global/documents/140041
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