Journal article
Exploring the virome of cattle with non-suppurative encephalitis of unknown etiology by metagenomics.
- Wüthrich D Interfaculty Bioinformatics Unit, University of Bern and Swiss Institute of Bioinformatics, Bern, Switzerland; Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland.
- Boujon CL Neurocenter, Division of Neurological Sciences, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
- Truchet L Neurocenter, Division of Neurological Sciences, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
- Selimovic-Hamza S Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland; Neurocenter, Division of Neurological Sciences, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
- Oevermann A Neurocenter, Division of Neurological Sciences, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
- Bouzalas IG Neurocenter, Division of Neurological Sciences, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
- Bruggmann R Interfaculty Bioinformatics Unit, University of Bern and Swiss Institute of Bioinformatics, Bern, Switzerland.
- Seuberlich T Neurocenter, Division of Neurological Sciences, Vetsuisse Faculty, University of Bern, Bern, Switzerland. Electronic address: torsten.seuberlich@vetsuisse.unibe.ch.
- 2016-03-21
Published in:
- Virology. - 2016
Astrovirus
Cattle
Encephalitis
Herpesvirus
Metagenomics
Neurological disease
Parainfluenza virus
Polyomavirus
Retrovirus
Animals
Case-Control Studies
Cattle
Cattle Diseases
DNA, Viral
Encephalitis, Viral
Metagenomics
RNA, Viral
Sequence Analysis, DNA
Sequence Analysis, RNA
Viruses
English
Non-suppurative encephalitis is one of the most frequent pathological diagnosis in cattle with neurological disease, but there is a gap in the knowledge on disease-associated pathogens. In order to identify viruses that are associated with non-suppurative encephalitis in cattle, we used a viral metagenomics approach on a sample set of 16 neurologically-diseased cows. We detected six virus candidates: parainfluenza virus 5 (PIV-5), bovine astrovirus CH13/NeuroS1 (BoAstV-CH13/NeuroS1), bovine polyomavirus 2 (BPyV-2 SF), ovine herpesvirus 2 (OvHV-2), bovine herpesvirus 6 (BHV-6) and a novel bovine betaretrovirus termed BoRV-CH15. In a case-control study using PCR, BoAstV-CH13 (p=0.046), BoPV-2 SF (p=0.005) and BoHV-6 (p=4.3E-05) were statistically associated with the disease. These data expand our knowledge on encephalitis-associated pathogens in cattle and point to the value of NGS in resolving complex infection scenarios in a clinical disease setting.
- Language
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- English
- Open access status
- hybrid
- Identifiers
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- DOI 10.1016/j.virol.2016.03.009
- PMID 26994586
- Persistent URL
- https://sonar.ch/global/documents/140316
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