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Novel in vitro diagnosis of equine allergies using a protein array and mathematical modelling approach: a proof of concept using insect bite hypersensitivity.
Journal article

Novel in vitro diagnosis of equine allergies using a protein array and mathematical modelling approach: a proof of concept using insect bite hypersensitivity.

  • Marti E Department of Clinical Research and Veterinary Public Health, University of Bern, Switzerland. Electronic address: eliane.marti@vetsuisse.unibe.ch.
  • Wang X School of Biosciences, University of Nottingham, Sutton Bonington campus, Loughborough LE12 5RD, UK.
  • Jambari NN School of Biosciences, University of Nottingham, Sutton Bonington campus, Loughborough LE12 5RD, UK.
  • Rhyner C Swiss Institute of Allergy and Asthma Research, University of Zürich, Davos, Switzerland.
  • Olzhausen J Swiss Institute of Allergy and Asthma Research, University of Zürich, Davos, Switzerland.
  • Pérez-Barea JJ School of Biosciences, University of Nottingham, Sutton Bonington campus, Loughborough LE12 5RD, UK.
  • Figueredo GP School of Computer Sciences, Advance Data Analysis Centre (ADAC), University of Nottingham, Jubilee Campus, Nottingham, UK.
  • Alcocer MJ School of Biosciences, University of Nottingham, Sutton Bonington campus, Loughborough LE12 5RD, UK.
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  • 2015-07-13
Published in:
  • Veterinary immunology and immunopathology. - 2015
Allergens
Chemometrics
Horse
IgE
Protein array
Allergens
Animals
Case-Control Studies
Ceratopogonidae
Diagnosis, Computer-Assisted
Female
Horse Diseases
Horses
Hypersensitivity
Immunoglobulin E
Insect Bites and Stings
Male
Mathematical Concepts
Models, Immunological
Protein Array Analysis
Skin
English Insect bite hypersensitivity (IBH) is a seasonal recurrent skin allergy of horses caused by IgE-mediated reactions to allergens present in the saliva of biting insects of the genus Culicoides, and possibly also Simulium and Stomoxys species. In this work we show that protein microarrays containing complex extracts and pure proteins, including recombinant Culicoides allergens, can be used as a powerful technique for the diagnosis of IBH. Besides the obvious advantages such as general profiling and use of few microliters of samples, this microarray technique permits automation and allows the generation of mathematical models with the calculation of individual risk profiles that can support the clinical diagnosis of allergic diseases. After selection of variables on influence on the projection (VIP), the observed values of sensitivity and specificity were 1.0 and 0.967, respectively. This confirms the highly discriminatory power of this approach for IBH and made it possible to attain a robust predictive mathematical model for this disease. It also further demonstrates the specificity of the protein array method on identifying a particular IgE-mediated disease when the sensitising allergen group is known.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/140428
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