Transcriptional regulatory framework for vascular cambium development in Arabidopsis roots.
- Zhang J Institute of Biotechnology, HiLIFE, University of Helsinki, Helsinki, Finland.
- Eswaran G Institute of Biotechnology, HiLIFE, University of Helsinki, Helsinki, Finland.
- Alonso-Serra J Institute of Biotechnology, HiLIFE, University of Helsinki, Helsinki, Finland.
- Kucukoglu M Institute of Biotechnology, HiLIFE, University of Helsinki, Helsinki, Finland.
- Xiang J Institute of Biotechnology, HiLIFE, University of Helsinki, Helsinki, Finland.
- Yang W The Sainsbury Laboratory, University of Cambridge, Cambridge, UK.
- Elo A Institute of Biotechnology, HiLIFE, University of Helsinki, Helsinki, Finland.
- Nieminen K Production Systems, Natural Resources Institute Finland (Luke), Helsinki, Finland.
- Damén T Institute of Biotechnology, HiLIFE, University of Helsinki, Helsinki, Finland.
- Joung JG Samsung Genome Institute, Samsung Medical Center, Seoul, South Korea.
- Yun JY Center for Genome Engineering, Institute for Basic Science, Daejeon, South Korea.
- Lee JH School of Biological Sciences, Seoul National University, Seoul, South Korea.
- Ragni L ZMBP-Center for Plant Molecular Biology, University of Tübingen, Tübingen, Germany.
- Barbier de Reuille P Institute of Plant Sciences, University of Bern, Bern, Switzerland.
- Ahnert SE The Sainsbury Laboratory, University of Cambridge, Cambridge, UK.
- Lee JY School of Biological Sciences, Seoul National University, Seoul, South Korea. jl924@snu.ac.kr.
- Mähönen AP Institute of Biotechnology, HiLIFE, University of Helsinki, Helsinki, Finland. aripekka.mahonen@helsinki.fi.
- Helariutta Y Institute of Biotechnology, HiLIFE, University of Helsinki, Helsinki, Finland. yrjo.helariutta@slcu.cam.ac.uk.
- 2019-10-10
Published in:
- Nature plants. - 2019
Arabidopsis
Cambium
Gene Expression Regulation, Plant
Genotype
Plant Development
Plant Roots
Transcription Factors
Transcription, Genetic
Transcriptome
English
Vascular cambium, a lateral plant meristem, is a central producer of woody biomass. Although a few transcription factors have been shown to regulate cambial activity1, the phenotypes of the corresponding loss-of-function mutants are relatively modest, highlighting our limited understanding of the underlying transcriptional regulation. Here, we use cambium cell-specific transcript profiling followed by a combination of transcription factor network and genetic analyses to identify 62 new transcription factor genotypes displaying an array of cambial phenotypes. This approach culminated in virtual loss of cambial activity when both WUSCHEL-RELATED HOMEOBOX 4 (WOX4) and KNOTTED-like from Arabidopsis thaliana 1 (KNAT1; also known as BREVIPEDICELLUS) were mutated, thereby unlocking the genetic redundancy in the regulation of cambium development. We also identified transcription factors with dual functions in cambial cell proliferation and xylem differentiation, including WOX4, SHORT VEGETATIVE PHASE (SVP) and PETAL LOSS (PTL). Using the transcription factor network information, we combined overexpression of the cambial activator WOX4 and removal of the putative inhibitor PTL to engineer Arabidopsis for enhanced radial growth. This line also showed ectopic cambial activity, thus further highlighting the central roles of WOX4 and PTL in cambium development.
- Language
-
- English
- Open access status
- green
- Identifiers
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- DOI 10.1038/s41477-019-0522-9
- PMID 31595065
- Persistent URL
- https://sonar.ch/global/documents/142322
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