Journal article
Affinity-capture reagents for protein arrays.
- Elia G Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland. giueli@pharma.anbi.ethz.ch
- Silacci M
- Scheurer S
- Scheuermann J
- Neri D
- 2003-02-07
Published in:
- Trends in biotechnology. - 2002
Biotechnology
Enzyme-Linked Immunosorbent Assay
Kinetics
Peptide Library
Peptides
Protein Array Analysis
Protein Binding
Proteins
Proteome
English
The simultaneous identification and quantitative measurement of the production levels of thousands of different proteins in a biological specimen remains an unachieved goal of modern proteomic research. Advances in the development of microarray-based platforms for highly parallel detection of proteins have therefore received a considerable impulse during the last few years. Here, we review the existing reagents for affinity capture of protein targets, as well as the techniques used for their immobilization on solid supports and methods for the detection of binding events, underlining the problems and the opportunities in this continuously evolving research field.
- Language
-
- English
- Open access status
- closed
- Identifiers
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- DOI 10.1016/s1471-1931(02)00201-x
- PMID 12570155
- Persistent URL
- https://sonar.ch/global/documents/142530
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