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Interaction of gap genes in the Drosophila head: tailless regulates expression of empty spiracles in early embryonic patterning and brain development.
Journal article

Interaction of gap genes in the Drosophila head: tailless regulates expression of empty spiracles in early embryonic patterning and brain development.

  • 2001-12-04
Published in:
  • Mechanisms of development. - 2001
Animals
Animals, Genetically Modified
Base Sequence
Binding Sites
Brain
DNA-Binding Proteins
Deoxyribonuclease I
Drosophila
Drosophila Proteins
Ectoderm
Enhancer Elements, Genetic
Genes, Reporter
Homeodomain Proteins
Immunohistochemistry
In Situ Hybridization
Microscopy, Confocal
Models, Genetic
Molecular Sequence Data
Mutation
Neurons
Repressor Proteins
Transcription, Genetic
Transgenes
English Unlike gap genes in the trunk region of Drosophila embryos, gap genes in the head were presumed not to regulate each other's transcription. Here, we show that in tailless (tll) loss-of-function mutants the empty spiracles (ems) expression domain in the head expands, whereas it retracts in tll gain-of-function embryos. We have identified a 304bp element in the ems-enhancer which is sufficient to drive expression in the head and brain and which contains two TLL and two BCD binding sites. Transgenic reporter gene lines containing mutations of the TLL binding sites demonstrate that tll directly inhibits the expression of ems in the early embryonic head and the protocerebral brain anlage. These results are the first demonstration of direct transcriptional regulation between gap genes in the head.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/142869
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