Journal article
Enzyme- and affinity biomolecule-mediated polymerization systems for biological signal amplification and cell screening.
- Malinowska KH Lehrstuhl für Angewandte Physik and Center for Nanoscience, Ludwig-Maximilians-Universität, 80799 Munich, Germany.
- Nash MA Lehrstuhl für Angewandte Physik and Center for Nanoscience, Ludwig-Maximilians-Universität, 80799 Munich, Germany; Department of Chemistry, University of Basel, 4056 Basel, Switzerland; Department of Biosystems Science and Engineering, Eidgenössische Technische Hochschule (ETH-Zürich), 4058 Basel, Switzerland. Electronic address: michael.nash@lmu.de.
- 2016-02-27
Published in:
- Current opinion in biotechnology. - 2016
Biosensing Techniques
Biotechnology
Enzymes
Macromolecular Substances
Polymerization
Signal Processing, Computer-Assisted
English
Enzyme-mediated polymerization and polymerization-based signal amplification have emerged as two closely related techniques that are broadly applicable in the nanobio sciences. We review recent progress on polymerization systems mediated by biological molecules (e.g., affinity molecules and enzymes), and highlight newly developed formats and configurations of these systems to perform such tasks as non-instrumented biodetection, synthesis of core-shell nanomaterials, isolation of rare cells, and high-throughput screening. We discuss useful features of biologically mediated polymerization systems, such as multiple mechanisms of amplification (e.g., enzymatic, radical chain propagation), and the ability to localize structures at interfaces and at cell surfaces with microscopic spatial confinement. We close with a perspective on desirable improvements that need to be addressed to adapt these molecular systems to future applications.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.1016/j.copbio.2016.01.007
- PMID 26919227
- Persistent URL
- https://sonar.ch/global/documents/143445
Statistics
Document views: 46
File downloads: