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Tape-Stripping Electrochemical Detection of Melanoma.
Journal article

Tape-Stripping Electrochemical Detection of Melanoma.

  • Darvishi S Laboratory of Physical and Analytical Electrochemistry , École Polytechnique Fédérale de Lausanne (EPFL) , Valais Wallis, Rue de l'Industrie 17 , CH-1950 Sion , Switzerland.
  • Pick H Institute of Chemical Sciences and Engineering , École Polytechnique Fédérale de Lausanne, EPFL , CH-1015 Lausanne , Switzerland.
  • Lin TE Institute of Biomedical Engineering, College of Electrical and Computer Engineering , National Chiao Tung University , Hsinchu 30010 , Taiwan.
  • Zhu Y Laboratory of Physical and Analytical Electrochemistry , École Polytechnique Fédérale de Lausanne (EPFL) , Valais Wallis, Rue de l'Industrie 17 , CH-1950 Sion , Switzerland.
  • Li X Department of Oncology , University of Lausanne , Ch. des Boveresses 155 , CH-1015 Epalinges , Switzerland.
  • Ho PC Department of Oncology , University of Lausanne , Ch. des Boveresses 155 , CH-1015 Epalinges , Switzerland.
  • Girault HH Laboratory of Physical and Analytical Electrochemistry , École Polytechnique Fédérale de Lausanne (EPFL) , Valais Wallis, Rue de l'Industrie 17 , CH-1950 Sion , Switzerland.
  • Lesch A Department of Industrial Chemistry "Toso Montanari" , University of Bologna , Viale del Risorgimento 4 , IT-40136 Bologna , Italy.
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  • 2019-09-19
Published in:
  • Analytical chemistry. - 2019
Adhesives
Animals
Antibodies, Monoclonal
Biomarkers, Tumor
Catalysis
Horseradish Peroxidase
Humans
Melanoma, Experimental
Mice
Microscopy, Electrochemical, Scanning
Monophenol Monooxygenase
Oxidation-Reduction
Skin Neoplasms
Tumor Cells, Cultured
English A noninvasive electrochemical melanoma detection approach based on using adhesive tapes for collecting and fixing cells from a suspicious skin area and transferring the cells into a scanning electrochemical microscope (SECM) is presented. The adhesive layer collects the cells reproducibly and keeps them well adhered on the tape during experiments in an electrolyte solution. A melanoma biomarker, here the intracellular enzyme tyrosinase (TYR), was imaged on the tape-collected cells without further cell lysing using antibodies that were labeled with horseradish peroxidase (HRP). The HRP labels catalyzed the oxidation of a dissolved redox-active species, which was detected at a soft microelectrode, gently brushed in contact mode over the tape. The melanoma biomarker was first detected on tape-stripped samples with murine melanoma cells of different concentrations. Thereafter, increasing levels of TYR were recorded in cells that were collected from the skin of melanoma mouse models representing three different stages of tumor growth. Additionally, SECM results of tape-stripped different human melanoma cell lines were confirmed by previous studies based on traditionally fixed and permeabilized cells.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/145006
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