Journal article
Localization of lysobisphosphatidic acid-rich membrane domains in late endosomes.
- Kobayashi T Department of Biochemistry, Science II, University of Geneva, Switzerland.
- Startchev K
- Whitney AJ
- Gruenber J
- 2001-05-12
Published in:
- Biological chemistry. - 2001
Animals
Antibodies, Monoclonal
Cell Line
Endosomes
Enzyme-Linked Immunosorbent Assay
Fluorescein-5-isothiocyanate
Fluorescent Antibody Technique
Intracellular Membranes
Lysophospholipids
Microinjections
Monoglycerides
English
Late endosomes accumulate internal membranes within the lumen of the organelle. These internal membranes are enriched in the late endosome specific phospholipid, lysobisphosphatidic acid (LBPA). The organization of LBPA-rich membrane domains is not well characterized. Using an LBPA-specific monoclonal antibody (6C4), we show that these membrane domains are not accessible from the cytoplasm. Using fluorescence correlation spectroscopy, we also show that 6C4 only binds sonicated, but not intact, late endosomes, presumably reflecting the release of internal membranes upon endosome rupture.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.1515/BC.2001.059
- PMID 11347897
- Persistent URL
- https://sonar.ch/global/documents/145693
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