Journal article
Residual polymerase activity-induced bias in terminal restriction fragment length polymorphism analysis.
- Hartmann M Molecular Ecology, Agroscope Reckenholz-Tänikon Research Station ART, CH-8046 Zurich, Switzerland.
- Enkerli J
- Widmer F
- 2007-01-16
Published in:
- Environmental microbiology. - 2007
Bacteria
DNA-Directed DNA Polymerase
Genotype
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Reproducibility of Results
Soil Microbiology
English
Residual activity of polymerase chain reaction DNA polymerases in restriction analyses strongly affected genetic profiling based on terminal restriction fragment length polymorphisms. Artificial fragment sizes produced as a result of 5'-overhang restriction site fill-in and addition of a terminal A may bias genetic profiling and genotyping of microbial communities. Efficient removal of polymerases retained original fragment sizes and significantly reduced this profiling bias in soil bacterial communities.
- Language
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- English
- Open access status
- closed
- Identifiers
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- DOI 10.1111/j.1462-2920.2006.01169.x
- PMID 17222153
- Persistent URL
- https://sonar.ch/global/documents/145961
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