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The eukaryotic way to defend and edit genomes by sRNA-targeted DNA deletion.
Journal article

The eukaryotic way to defend and edit genomes by sRNA-targeted DNA deletion.

  • Swart EC Institute of Cell Biology, University of Bern, Bern, Switzerland.
  • Nowacki M
  • 2015-01-13
Published in:
  • Annals of the New York Academy of Sciences. - 2015
CRISPR/Cas
DNA excision
IES/Ias
genome editing
sRNA
sRNA-targeted DNA deletion
Archaea
Bacteria
Clustered Regularly Interspaced Short Palindromic Repeats
DNA
Eukaryota
Evolution, Molecular
Genome
Models, Genetic
RNA, Small Untranslated
Sequence Deletion
English While there is currently burgeoning interest in the application of the CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated genes) to genome editing, it is perhaps not widely appreciated that this is the second discovery of a small RNA (sRNA)-targeted DNA-deletion system. The first sRNA-targeted DNA-deletion system to be discovered, which we call IES/Ias (internal eliminated sequence/IES-associated genes) to contrast with CRISPR/Cas, is found in ciliates, and, like CRISPR/Cas, is thought to serve as a form of immune defense against invasive DNAs. The manner in which the ciliate IES/Ias system functions is distinct from that of the CRISPR/Cas system in archaea and bacteria, and arose independently through a synthesis of RNA interference-derived and DNA-specific molecular components. Despite the major differences between CRISPR/Cas and IES/Ias, both systems face similar conceptual challenges in targeting invasive DNAs. In this review, we focus on the discovery, effects, function, and evolutionary consequences of the IES/Ias system.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/14631
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