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Sequestration of host metabolism by an intracellular pathogen
Journal article

Sequestration of host metabolism by an intracellular pathogen

  • Gehre, Lena CNRS UMR3691, Paris, France
  • Gorgette, Olivier Plate-forme de Microscopie Ultrastructurale, Imagopole, Institut Pasteur, Paris, France
  • Perrinet, Stéphanie CNRS UMR3691, Paris, France
  • Prevost, Marie-Christine Plate-forme de Microscopie Ultrastructurale, Imagopole, Institut Pasteur, Paris, France
  • Ducatez, Mathieu Unité de Glycobiologie Structurale et Fonctionnelle - CNRS UMR8576, Université de Lille, Lille, France
  • Giebel, Amanda M Department of Biology, Indiana University Bloomington, Bloomington, United States
  • Nelson, David E Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, United States
  • Ball, Steven G Unité de Glycobiologie Structurale et Fonctionnelle - CNRS UMR8576, Université de Lille, Lille, France
  • Subtil, Agathe CNRS UMR3691, Paris, France
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  • 2016-3-16
Published in:
  • eLife. - eLife Sciences Publications, Ltd. - 2016, vol. 5
English For intracellular pathogens, residence in a vacuole provides a shelter against cytosolic host defense to the cost of limited access to nutrients. The human pathogen Chlamydia trachomatis grows in a glycogen-rich vacuole. How this large polymer accumulates there is unknown. We reveal that host glycogen stores shift to the vacuole through two pathways: bulk uptake from the cytoplasmic pool, and de novo synthesis. We provide evidence that bacterial glycogen metabolism enzymes are secreted into the vacuole lumen through type 3 secretion. Our data bring strong support to the following scenario: bacteria co-opt the host transporter SLC35D2 to import UDP-glucose into the vacuole, where it serves as substrate for de novo glycogen synthesis, through a remarkable adaptation of the bacterial glycogen synthase. Based on these findings we propose that parasitophorous vacuoles not only offer protection but also provide a microorganism-controlled metabolically active compartment essential for redirecting host resources to the pathogens.
Language
  • English
Open access status
gold
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Persistent URL
https://sonar.ch/global/documents/146353
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