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HSV-1 amplicon vectors as genetic vaccines.
Journal article

HSV-1 amplicon vectors as genetic vaccines.

  • Laimbacher AS Vetsuisse Faculty, Institute of Virology, University of Zurich, Winterthurerstrasse 266a, 8057, Zurich, Switzerland.
  • Fraefel C
  • 2014-03-28
Published in:
  • Methods in molecular biology (Clifton, N.J.). - 2014
Animals
Chlorocebus aethiops
Foot-and-Mouth Disease
Foot-and-Mouth Disease Virus
Herpesvirus 1, Human
Humans
Mice
Molecular Biology
Rotavirus
Rotavirus Infections
Vero Cells
Viral Structural Proteins
Viral Vaccines
English HSV-1 amplicon vectors have been used as platforms for the generation of genetic vaccines against both DNA and RNA viruses. Mice vaccinated with such vectors encoding structural proteins from both foot-and-mouth disease virus and rotavirus were partially protected from challenge with wild-type virus (D'Antuono et al. Vaccine 28: 7363-7372, 2010; Laimbacher et al. Mol Ther 20: 1810-1820, 2012), indicating that HSV-1 amplicon vectors are attractive tools for the development of complex and safe genetic vaccines. This chapter describes the use of HSV-1 amplicon vectors that encode individual or multiple viral structural proteins from a polycistronic transgene cassette in mammalian cells. More precisely, amplicon vectors that encode multiple structural viral proteins support the in situ production of viruslike particles (VLPs) in vector-infected cells. The expression of the viral genes is confirmed by Western blot and immune fluorescence analysis, and the generation of VLPs in vector-infected cells is demonstrated by electron microscopy.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/147157
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