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Molecular characterization of chromosome termini of the arbuscular mycorrhizal fungus Glomus intraradices (Glomeromycota).
Journal article

Molecular characterization of chromosome termini of the arbuscular mycorrhizal fungus Glomus intraradices (Glomeromycota).

  • Hijri M Department of Ecology and Evolution, Biology Building, University of Lausanne, 1015 Lausanne, Switzerland. Mohamed.Hijri@umontreal.ca
  • Niculita H
  • Sanders IR
  • 2007-05-01
Published in:
  • Fungal genetics and biology : FG & B. - 2007
Chromosomes, Fungal
DNA, Fungal
Electrophoresis, Gel, Pulsed-Field
Genome, Fungal
Mycorrhizae
Phylogeny
Polymerase Chain Reaction
Telomere
English The minimum chromosome number of Glomus intraradices was assessed through cloning and sequencing of the highly divergent telomere-associated sequences (TAS) and by pulsed field gel electrophoresis (PFGE). The telomere of G. intraradices, as in other filamentous fungi, consists of TTAGGG repeats, this was confirmed using Bal31 nuclease time course reactions. Telomere length was estimated to be roughly 0.9 kb by Southern blots on genomic DNA and a telomere probe. We have identified six classes of cloned chromosomal termini based on the TAS. An unusually high genetic variation was observed within two of the six TAS classes. To further assess the total number of chromosome termini, we used telomere fingerprinting. Surprisingly, all hybridization patterns showed smears, which demonstrate that TAS are remarkably variable in the G. intraradices genome. These analyses predict the presence of at least three chromosomes in G. intraradices while PFGE showed a pattern of four bands ranging from 1.2 to 1.5 Mb. Taken together, our results indicate that there are at least four chromosomes in G. intraradices but there are probably more. The information on TAS and telomeres in the G. intradicies will be essential for making a physical map of the G. intraradices genome and could provide molecular markers for future studies of genetic variation among nuclei in these multigenomic fungi.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/147695
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