Developmental and comparative transcriptomic identification of iridophore contribution to white barring in clownfish.
- Salis P Observatoire Océanologique de Banyuls-sur-Mer, UMR CNRS 7232 BIOM, Sorbonne Université, Banyuls-sur-Mer, France.
- Lorin T IGFL, ENS de Lyon, UMR 5242 CNRS, Université Claude Bernard Lyon I, Lyon Cedex 07, France.
- Lewis V Department of Biology, University of Washington, Seattle, Washington.
- Rey C ENS de Lyon, CNRS UMR 5239, INSERM U1210, LBMC, Université Claude Bernard, Lyon, France.
- Marcionetti A Department of Computational Biology, Biophore, University of Lausanne, Lausanne, Switzerland.
- Escande ML Observatoire Océanologique de Banyuls-sur-Mer, UMR CNRS 7232 BIOM, Sorbonne Université, Banyuls-sur-Mer, France.
- Roux N Observatoire Océanologique de Banyuls-sur-Mer, UMR CNRS 7232 BIOM, Sorbonne Université, Banyuls-sur-Mer, France.
- Besseau L Observatoire Océanologique de Banyuls-sur-Mer, UMR CNRS 7232 BIOM, Sorbonne Université, Banyuls-sur-Mer, France.
- Salamin N Department of Computational Biology, Biophore, University of Lausanne, Lausanne, Switzerland.
- Sémon M ENS de Lyon, CNRS UMR 5239, INSERM U1210, LBMC, Université Claude Bernard, Lyon, France.
- Parichy D Department of Biology, Department of Cell Biology, University of Virginia, Charlottesville, Virginia.
- Volff JN IGFL, ENS de Lyon, UMR 5242 CNRS, Université Claude Bernard Lyon I, Lyon Cedex 07, France.
- Laudet V Observatoire Océanologique de Banyuls-sur-Mer, UMR CNRS 7232 BIOM, Sorbonne Université, Banyuls-sur-Mer, France.
- 2019-01-12
Published in:
- Pigment cell & melanoma research. - 2019
Amphiprion
clownfish
coral reef fish
iridophore
leucophore
transcriptomic
Animals
Chromatophores
Fish Proteins
Fishes
Gene Expression Regulation, Developmental
Genome
Pigmentation
Transcriptome
English
Actinopterygian fishes harbor at least eight distinct pigment cell types, leading to a fascinating diversity of colors. Among this diversity, the cellular origin of the white color appears to be linked to several pigment cell types such as iridophores or leucophores. We used the clownfish Amphiprion ocellaris, which has a color pattern consisting of white bars over a darker body, to characterize the pigment cells that underlie the white hue. We observe by electron microscopy that cells in white bars are similar to iridophores. In addition, the transcriptomic signature of clownfish white bars exhibits similarities with that of zebrafish iridophores. We further show by pharmacological treatments that these cells are necessary for the white color. Among the top differentially expressed genes in white skin, we identified several genes (fhl2a, fhl2b, saiyan, gpnmb, and apoD1a) and show that three of them are expressed in iridophores. Finally, we show by CRISPR/Cas9 mutagenesis that these genes are critical for iridophore development in zebrafish. Our analyses provide clues to the genomic underpinning of color diversity and allow identification of new iridophore genes in fish.
- Language
-
- English
- Open access status
- green
- Identifiers
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- DOI 10.1111/pcmr.12766
- PMID 30633441
- Persistent URL
- https://sonar.ch/global/documents/149150
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