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Metabolic activity of mature biofilms of Mycobacterium tuberculosis and other non-tuberculous mycobacteria.

  • Solokhina A Center of Biomechanics & Biocalorimetry, University Basel, Gewerbestr. 14, CH-4123, Allschwil, Switzerland.
  • Brückner D Center of Biomechanics & Biocalorimetry, University Basel, Gewerbestr. 14, CH-4123, Allschwil, Switzerland.
  • Bonkat G Alta Uro AG, Centralbahnplatz 6, CH-4051, Basel, Switzerland.
  • Braissant O Center of Biomechanics & Biocalorimetry, University Basel, Gewerbestr. 14, CH-4123, Allschwil, Switzerland. Olivier.braissant@unibas.ch.
  • 2017-08-25
Published in:
  • Scientific reports. - 2017
Biofilms
Carbon Dioxide
Energy Metabolism
Mycobacterium tuberculosis
Nontuberculous Mycobacteria
Oxygen
English Mycobacteria are classified into two groups, fast- and slow-growing. Often, fast-growing mycobacteria are assumed to have a higher metabolic activity than their slower counterparts, but in mature biofilms this assumption might not be correct. Indeed, when measuring the metabolic activity of mycobacterial biofilms with two independent non-invasive techniques (isothermal microcalorimetry and tunable diode laser absorption spectrometry), mature biofilms of slow- and fast-growing species appeared more alike than expected. Metabolic heat production rate was 2298 ± 181 µW for M. smegmatis and 792 ± 81 µW for M. phlei, while M. tuberculosis and M. bovis metabolic heat production rates were between these values. These small differences were further confirmed by similar oxygen consumption rates (3.3 ± 0.2 nMole/s and 1.7 ± 0.3 nMole/s for M. smegmatis and M. tuberculosis, respectively). These data suggest that the metabolic potential of slow-growing mycobacterial biofilms has been underestimated, particularly for pathogenic species.
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  • English
Open access status
gold
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https://sonar.ch/global/documents/150179
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