Identification of single nucleotides in MoS2 nanopores.
- Feng J Laboratory of Nanoscale Biology, Institute of Bioengineering, School of Engineering, EPFL, Lausanne 1015, Switzerland.
- Liu K Laboratory of Nanoscale Biology, Institute of Bioengineering, School of Engineering, EPFL, Lausanne 1015, Switzerland.
- Bulushev RD Laboratory of Nanoscale Biology, Institute of Bioengineering, School of Engineering, EPFL, Lausanne 1015, Switzerland.
- Khlybov S Laboratory of Nanoscale Biology, Institute of Bioengineering, School of Engineering, EPFL, Lausanne 1015, Switzerland.
- Dumcenco D Laboratory of Nanoscale Electronics and Structure, Institute of Electrical Engineering, School of Engineering, EPFL, Lausanne 1015, Switzerland.
- Kis A Laboratory of Nanoscale Electronics and Structure, Institute of Electrical Engineering, School of Engineering, EPFL, Lausanne 1015, Switzerland.
- Radenovic A Laboratory of Nanoscale Biology, Institute of Bioengineering, School of Engineering, EPFL, Lausanne 1015, Switzerland.
- 2015-09-22
Published in:
- Nature nanotechnology. - 2015
English
The size of the sensing region in solid-state nanopores is determined by the size of the pore and the thickness of the pore membrane, so ultrathin membranes such as graphene and single-layer molybdenum disulphide could potentially offer the necessary spatial resolution for nanopore DNA sequencing. However, the fast translocation speeds (3,000-50,000 nt ms(-1)) of DNA molecules moving across such membranes limit their usability. Here, we show that a viscosity gradient system based on room-temperature ionic liquids can be used to control the dynamics of DNA translocation through MoS2 nanopores. The approach can be used to statistically detect all four types of nucleotide, which are identified according to current signatures recorded during their transient residence in the narrow orifice of the atomically thin MoS2 nanopore. Our technique, which exploits the high viscosity of room-temperature ionic liquids, provides optimal single nucleotide translocation speeds for DNA sequencing, while maintaining a signal-to-noise ratio higher than 10.
- Language
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- English
- Open access status
- green
- Identifiers
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- DOI 10.1038/nnano.2015.219
- PMID 26389660
- Persistent URL
- https://sonar.ch/global/documents/151073
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