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Basic helix-loop-helix protein MyoD displays modest DNA binding specificity.
Journal article

Basic helix-loop-helix protein MyoD displays modest DNA binding specificity.

  • 1996-08-05
Published in:
  • FEBS letters. - 1996
Amino Acid Sequence
Animals
Base Sequence
Circular Dichroism
DNA
DNA-Binding Proteins
Helix-Loop-Helix Motifs
Kinetics
Macromolecular Substances
Mice
Molecular Sequence Data
Mutagenesis, Site-Directed
MyoD Protein
Oligodeoxyribonucleotides
Peptide Fragments
Point Mutation
Recombinant Proteins
English The expression of MyoD can activate muscle specific genes and myogenic differentiation in many cell types. The hypothesis that the DNA binding specificity of MyoD is responsible for its biological specificity was tested. Homodimers of MyoD bind to E-box containing DNA with high affinity, but do not form stable and well defined complexes with heterologous DNA sequences. The physiologically active heterodimer of MyoD and E12 binds an oligonucleotide containing an E-box sequence with an affinity only two orders of magnitude higher than a completely unrelated DNA sequence, stressing the importance of cooperative interactions with other proteins of the transcriptional machinery for specific gene activation.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/151846
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