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E-selectin binding promotes neutrophil activation in vivo in E-selectin transgenic mice.
Journal article

E-selectin binding promotes neutrophil activation in vivo in E-selectin transgenic mice.

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  • 1996-07-25
Published in:
  • Biochemical and biophysical research communications. - 1996
Actins
Animals
Base Sequence
Bone Marrow
Bone Marrow Cells
E-Selectin
Fluorescence
Liver
Lymphocyte Activation
Mice
Mice, Transgenic
Molecular Sequence Data
Muscles
Myocardium
Neutrophil Activation
Neutrophils
Promoter Regions, Genetic
Protein Binding
RNA, Messenger
English E-selectin is a membrane protein expressed by endothelial cells activated by cytokines released during the inflammatory process; it plays an important role in neutrophil emigration into inflamed tissues. To further explore in vivo the function of E-selectin, we have generated transgenic mouse line expressing E-selectin under the control of a chicken beta-actin promoter. In these mice, the number of blood neutrophils was reduced, without any other obvious phenotype or tissue damage. These neutrophils, however, displayed two significant changes: first, an alteration in the levels of expression of two membrane receptors involved in neutrophil adhesion to endothelial cells, namely a marked increased in the Mac-1 antigen (CD11b/CD18) and a decrease in the Mel-14 antigen (L-selectin); second, an increased oxidative activity when compared to blood neutrophils of non-transgenic mice, as shown by their capacity to oxidize 2',7'-dichlorofluorescein (DCFH) into a fluorescent compound. These observations indicate that the binding of E-selection with neutrophils bearing its ligands promotes neutrophil activation in vivo.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/152405
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