Journal article
Solute carrier family 12 member 2 as a proteomic and histological biomarker of dysplasia and neoplasia in ulcerative colitis.
- Merli AM Laboratory of Translational Gastroenterology, University of Liège, Liège, Belgium.
- Vieujean S Laboratory of Translational Gastroenterology, University of Liège, Liège, Belgium.
- Massot C Laboratory of Translational Gastroenterology, University of Liège, Liège, Belgium.
- Blétard N Pathological Anatomy and Cytology, University Hospital CHU of Liège, Liège, Belgium.
- Quesada Calvo F Laboratory of Translational Gastroenterology, University of Liège, Liège, Belgium.
- Baiwir D GIGA Proteomics Facility, University of Liège, Liège, Belgium.
- Mazzucchelli G Laboratory of Mass Spectrometry, University of Liège, Liège, Belgium.
- Servais L Laboratory of Cardiology, GIGA-Cardiovascular Sciences, University of Liège, Liège, Belgium.
- Wéra O Laboratory of Cardiology, GIGA-Cardiovascular Sciences, University of Liège, Liège, Belgium.
- Oury C Laboratory of Cardiology, GIGA-Cardiovascular Sciences, University of Liège, Liège, Belgium.
- de Leval L Institute of Pathology, Centre Hospitalier Universitaire Vaudois, University of Lausanne, Lausanne, Switzerland.
- Sempoux C Institute of Pathology, Centre Hospitalier Universitaire Vaudois, University of Lausanne, Lausanne, Switzerland.
- Manzini R Department of Gastroenterology and Hepatology, University Hospital Zurich, University of Zurich, Zurich, Switzerland.
- Bluemel S Department of Gastroenterology and Hepatology, University Hospital Zurich, University of Zurich, Zurich, Switzerland.
- Scharl M Department of Gastroenterology and Hepatology, University Hospital Zurich, University of Zurich, Zurich, Switzerland.
- Rogler G Department of Gastroenterology and Hepatology, University Hospital Zurich, University of Zurich, Zurich, Switzerland.
- De Pauw E Laboratory of Mass Spectrometry, University of Liège, Liège, Belgium.
- Coimbra Marques C Abdominal Surgery Department, University Hospital CHU of Liège, Liège, Belgium.
- Colard A Department of Gastroenterology, CHC Clinique Saint-Joseph, Liège, Belgium.
- Vijverman A Department of Gastroenterology, CHR Citadelle, Liège, Belgium.
- Delvenne P Pathological Anatomy and Cytology, University Hospital CHU of Liège, Liège, Belgium.
- Louis E Laboratory of Translational Gastroenterology, University of Liège, Liège, Belgium.
- Meuwis MA Laboratory of Translational Gastroenterology, University of Liège, Liège, Belgium.
- 2020-09-13
Published in:
- Journal of Crohn's & colitis. - 2020
English
BACKGROUND AND AIMS
Ulcerative colitis (UC) patients have a greater risk of developing colorectal cancer through inflammation-dysplasia-carcinoma sequence of transformation. The histopathological diagnosis of dysplasia is therefore of critical clinical relevance, but dysplasia may be difficult to distinguish from inflammatory changes.
METHODS
A proteomic pilot study on 5 UC colorectal dysplastic patients highlighted proteins differentially distributed between paired dysplastic, inflammatory and normal tissues. The best candidate marker was selected and immunohistochemistry confirmation was performed on AOM/DSS mouse model lesions, 37 UC dysplasia, 14 UC cancers, 23 longstanding UC, 35 sporadic conventional adenomas, 57 sporadic serrated lesions and 82 sporadic colorectal cancers.
RESULTS
Differential proteomics found 11 proteins significantly more abundant in dysplasia compared to inflammation, including Solute carrier family 12 member 2 (SLC12A2) which was confidently identified with 8 specific peptides and was below the limit of quantitation in both inflammatory and normal colon. SLC12A2 immunohistochemical analysis confirmed the discrimination of preneoplastic and neoplastic lesions from inflammatory lesions in mice, UC and in sporadic contexts. A specific SLC12A2 staining pattern termed "loss of gradient" reached 89% sensitivity, 95% specificity and 92% accuracy for UC-dysplasia diagnosis together with an inter-observer agreement of 95.24% (multirater κfree of 0.90; IC95%: 0.78 - 1.00). Such discrimination could not be obtained by Ki67 staining. This specific pattern was also associated with sporadic colorectal adenomas and cancers.
CONCLUSIONS
We found a specific SLC12A2 immunohistochemical staining pattern in precancerous and cancerous colonic UC-lesions which could be helpful for diagnosing dysplasia and cancer in UC and non-UC patients.
Ulcerative colitis (UC) patients have a greater risk of developing colorectal cancer through inflammation-dysplasia-carcinoma sequence of transformation. The histopathological diagnosis of dysplasia is therefore of critical clinical relevance, but dysplasia may be difficult to distinguish from inflammatory changes.
METHODS
A proteomic pilot study on 5 UC colorectal dysplastic patients highlighted proteins differentially distributed between paired dysplastic, inflammatory and normal tissues. The best candidate marker was selected and immunohistochemistry confirmation was performed on AOM/DSS mouse model lesions, 37 UC dysplasia, 14 UC cancers, 23 longstanding UC, 35 sporadic conventional adenomas, 57 sporadic serrated lesions and 82 sporadic colorectal cancers.
RESULTS
Differential proteomics found 11 proteins significantly more abundant in dysplasia compared to inflammation, including Solute carrier family 12 member 2 (SLC12A2) which was confidently identified with 8 specific peptides and was below the limit of quantitation in both inflammatory and normal colon. SLC12A2 immunohistochemical analysis confirmed the discrimination of preneoplastic and neoplastic lesions from inflammatory lesions in mice, UC and in sporadic contexts. A specific SLC12A2 staining pattern termed "loss of gradient" reached 89% sensitivity, 95% specificity and 92% accuracy for UC-dysplasia diagnosis together with an inter-observer agreement of 95.24% (multirater κfree of 0.90; IC95%: 0.78 - 1.00). Such discrimination could not be obtained by Ki67 staining. This specific pattern was also associated with sporadic colorectal adenomas and cancers.
CONCLUSIONS
We found a specific SLC12A2 immunohistochemical staining pattern in precancerous and cancerous colonic UC-lesions which could be helpful for diagnosing dysplasia and cancer in UC and non-UC patients.
- Language
-
- English
- Open access status
- closed
- Identifiers
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- DOI 10.1093/ecco-jcc/jjaa168
- PMID 32920643
- Persistent URL
- https://sonar.ch/global/documents/153081
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