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Proteins and Signaling Pathways Response to Dry Needling Combined with Static Stretching Treatment for Chronic Myofascial Pain in a RAT Model: An Explorative Proteomic Study.

  • Li L Department of Sport Medicine and Rehabilitation Center, Shanghai University of Sport, Shanghai 200438, China. 1610302015@student.sus.edu.cn.
  • Huang Q Department of Sport Medicine and Rehabilitation Center, Shanghai University of Sport, Shanghai 200438, China. huaqia404@aliyun.com.
  • Barbero M Rehabilitation Research Laboratory 2rLab, Department of Business Economics Health and Social Care, University of Applied Sciences and Arts of Southern Switzerland, 6928 Manno, Switzerland. marco.barbero@supsi.ch.
  • Liu L Sport and Health Science Department, Nanjing Sport Institute, Nanjing 210014, China. liulinst1989@126.com.
  • Nguyen T Faculty of Sport Science, Ton Duc Thang University, Ho Chi Minh City 71000, Viet Nam. nguyenthitham@tdtu.edu.vn.
  • Xu A Department of Sport Medicine and Rehabilitation Center, Shanghai University of Sport, Shanghai 200438, China. 1620325008@student.sus.edu.cn.
  • Ji L Department of Sport Medicine and Rehabilitation Center, Shanghai University of Sport, Shanghai 200438, China. 1620325009@student.sus.edu.cn.
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  • 2019-02-01
Published in:
  • International journal of molecular sciences. - 2019
actinin alpha 3
calsequestrin-1
chronic myofascial pain
dry needling
mass spectrometry
myofascial trigger points
parvalbumin alpha
proteomic
stretching
tandem mass tag
Acupuncture Therapy
Animals
Chromatography, Liquid
Male
Muscle Stretching Exercises
Myofascial Pain Syndromes
Pain Measurement
Proteomics
Rats
Rats, Sprague-Dawley
Tandem Mass Spectrometry
English A quantitative proteomic analysis of the response to dry needling combined with static stretching treatment was performed in a rat model of active myofascial trigger points (MTrPs). 36 rats were divided into a model group (MG), a stretching group (SG) and a dry needling combined with stretching group (SDG). We performed three biological replicates to compare large-scale differential protein expression between groups by tandem mass tag (TMT) labeling technology based on nanoscale liquid chromatography mass spectrometry analysis (LC⁻MS/MS). Hierarchical clustering, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment and protein-protein interaction network analyses were performed for the general characterization of overall enriched proteins. For validation of the results of TMT, the candidate proteins were verified by parallel reaction monitoring (PRM) analysis. 285 differentially expressed proteins between groups were identified and quantified. Tight junction pathway played a dominant role in dry needling combined with static stretching treatment for the rat model of active MTrPs. Three candidate proteins, namely actinin alpha 3, calsequestrin-1 and parvalbumin alpha, were further validated, consistent with the results of LC⁻MS/MS. This is the first proteomics-based study to report the therapeutic mechanism underlying dry needling and static stretching treatment for MTrPs. Further functional verification of the potential signaling pathways and the enriched proteins is warranted.
Language
  • English
Open access status
gold
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Persistent URL
https://sonar.ch/global/documents/154366
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