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Focal molography is a new method for the in situ analysis of molecular interactions in biological samples.
Journal article

Focal molography is a new method for the in situ analysis of molecular interactions in biological samples.

  • Gatterdam V Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zurich, 8092 Zurich, Switzerland.
  • Frutiger A Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zurich, 8092 Zurich, Switzerland.
  • Stengele KP Roche Diagnostics GmbH, 82377 Penzberg, Germany.
  • Heindl D Roche Diagnostics GmbH, 82377 Penzberg, Germany.
  • Lübbers T Roche Pharma Research and Early Development, Roche Innovation Center Basel, 4070 Basel, Switzerland.
  • Vörös J Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zurich, 8092 Zurich, Switzerland.
  • Fattinger C Roche Pharma Research and Early Development, Roche Innovation Center Basel, 4070 Basel, Switzerland.
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  • 2017-09-26
Published in:
  • Nature nanotechnology. - 2017
Antibodies
Biosensing Techniques
Dynamic Light Scattering
Humans
Lab-On-A-Chip Devices
Lasers
Nanoparticles
English Focal molography is a next-generation biosensor that visualizes specific biomolecular interactions in real time. It transduces affinity modulation on the sensor surface into refractive index modulation caused by target molecules that are bound to a precisely assembled nanopattern of molecular recognition sites, termed the 'mologram'. The mologram is designed so that laser light is scattered at specifically bound molecules, generating a strong signal in the focus of the mologram via constructive interference, while scattering at nonspecifically bound molecules does not contribute to the effect. We present the realization of molograms on a chip by submicrometre near-field reactive immersion lithography on a light-sensitive monolithic graft copolymer layer. We demonstrate the selective and sensitive detection of biomolecules, which bind to the recognition sites of the mologram in various complex biological samples. This allows the label-free analysis of non-covalent interactions in complex biological samples, without a need for extensive sample preparation, and enables novel time- and cost-saving ways of performing and developing immunoassays for diagnostic tests.
Language
  • English
Open access status
closed
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Persistent URL
https://sonar.ch/global/documents/155026
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