IVIG regulates the survival of human but not mouse neutrophils.
- Schneider C Institute of Pharmacology, University of Bern, Bern, Switzerland.
- Wicki S Institute of Pharmacology, University of Bern, Bern, Switzerland.
- Graeter S Institute of Pharmacology, University of Bern, Bern, Switzerland.
- Timcheva TM Institute of Pharmacology, University of Bern, Bern, Switzerland.
- Keller CW Institute of Experimental Immunology, Laboratory of Neuroinflammation, University of Zurich, Zurich, Switzerland.
- Quast I Institute of Experimental Immunology, Laboratory of Neuroinflammation, University of Zurich, Zurich, Switzerland.
- Leontyev D Department of Medicine, University of Toronto and Centre for Innovation, Canadian Blood Services, Toronto, Ontario, Canada.
- Djoumerska-Alexieva IK Department of Immunology, Stefan Angelov Institute of Microbiology, Bulgarian Academy of Sciences, Sofia, Bulgaria.
- Käsermann F CSL Behring, Research, Bern, Switzerland.
- Jakob SM Department of Intensive Care Medicine, University Hospital Bern (Inselspital), University of Bern, Bern, Switzerland.
- Dimitrova PA Department of Immunology, Stefan Angelov Institute of Microbiology, Bulgarian Academy of Sciences, Sofia, Bulgaria.
- Branch DR Department of Medicine, University of Toronto and Centre for Innovation, Canadian Blood Services, Toronto, Ontario, Canada.
- Cummings RD Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
- Lünemann JD Institute of Experimental Immunology, Laboratory of Neuroinflammation, University of Zurich, Zurich, Switzerland.
- Kaufmann T Institute of Pharmacology, University of Bern, Bern, Switzerland.
- Simon HU Institute of Pharmacology, University of Bern, Bern, Switzerland.
- von Gunten S Institute of Pharmacology, University of Bern, Bern, Switzerland. stephan.vongunten@pki.unibe.ch.
- 2017-05-04
Published in:
- Scientific reports. - 2017
Animals
Antibodies, Monoclonal
Antigens, CD
Cell Survival
Humans
Immunoglobulin Fc Fragments
Immunoglobulin G
Immunoglobulins, Intravenous
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Neutrophils
Sialic Acid Binding Immunoglobulin-like Lectins
fas Receptor
English
Intravenous immunoglobulin (IVIG) are purified IgG preparations made from the pooled plasma from thousands of healthy donors and are being tested in preclinical mouse models. Inherent challenges, however, are the pluripotency of IVIG and its xenogeneicity in animals. IVIG can alter the viability of human neutrophils via agonistic antibodies to Fas and Siglec-9. In this study, we compared the effects of IVIG on human and mouse neutrophils using different death assays. Different commercial IVIG preparations similarly induced cytokine-dependent death in human neutrophils, whereas they had no effects on the survival of either peripheral blood or bone marrow neutrophils from C57BL/6 or BALB/c mice. F(ab')2 but not Fc fragments of IVIG induced death of human neutrophils, whereas neither of these IVIG fragments, nor agonistic monoclonal antibodies to human Fas or Siglec-9 affected the viability of mouse neutrophils. Pooled mouse IgG, which exhibited a different immunoprofile compared to IVIG, also had no effect on mouse cells. Together, these observations demonstrate that effects of IVIG on neutrophil survival are not adequately reflected in current mouse models, despite the key role of these cells in human inflammatory and autoimmune diseases.
- Language
-
- English
- Open access status
- gold
- Identifiers
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- DOI 10.1038/s41598-017-01404-0
- PMID 28465620
- Persistent URL
- https://sonar.ch/global/documents/155447
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