Protective immunity against infection with Mycoplasma haemofelis.
- Hicks CA School of Veterinary Sciences, University of Bristol, Bristol, United Kingdom Center for Clinical Studies, Vetsuisse Facility, University of Zurich, Zurich, Switzerland.
- Willi B Clinical Laboratory, Vetsuisse Facility, University of Zurich, Zurich, Switzerland Clinic for Small Animal Internal Medicine, Vetsuisse Facility, University of Zurich, Zurich, Switzerland.
- Riond B Clinical Laboratory, Vetsuisse Facility, University of Zurich, Zurich, Switzerland.
- Novacco M Clinical Laboratory, Vetsuisse Facility, University of Zurich, Zurich, Switzerland Center for Clinical Studies, Vetsuisse Facility, University of Zurich, Zurich, Switzerland.
- Meli ML Clinical Laboratory, Vetsuisse Facility, University of Zurich, Zurich, Switzerland Center for Clinical Studies, Vetsuisse Facility, University of Zurich, Zurich, Switzerland.
- Stokes CR School of Veterinary Sciences, University of Bristol, Bristol, United Kingdom.
- Helps CR School of Veterinary Sciences, University of Bristol, Bristol, United Kingdom.
- Hofmann-Lehmann R Clinical Laboratory, Vetsuisse Facility, University of Zurich, Zurich, Switzerland Center for Clinical Studies, Vetsuisse Facility, University of Zurich, Zurich, Switzerland.
- Tasker S School of Veterinary Sciences, University of Bristol, Bristol, United Kingdom S.Tasker@bristol.ac.uk.
- 2014-11-21
Published in:
- Clinical and vaccine immunology : CVI. - 2015
Animals
Antibodies, Bacterial
Bacterial Load
Blood
Cat Diseases
Cats
Cytokines
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Gene Expression Profiling
HSP70 Heat-Shock Proteins
Lymphocytes
Male
Mycoplasma
Mycoplasma Infections
Real-Time Polymerase Chain Reaction
English
Hemoplasmas are potentially zoonotic mycoplasmal pathogens, which are not consistently cleared by antibiotic therapy. Mycoplasma haemofelis is the most pathogenic feline hemoplasma species. The aim of this study was to determine how cats previously infected with M. haemofelis that had recovered reacted when rechallenged with M. haemofelis and to characterize the immune response following de novo M. haemofelis infection and rechallenge. Five specific-pathogen-free (SPF)-derived naive cats (group A) and five cats that had recovered from M. haemofelis infection (group B) were inoculated subcutaneously with M. haemofelis. Blood M. haemofelis loads were measured by quantitative PCR (qPCR), antibody response to heat shock protein 70 (DnaK) by enzyme-linked immunosorbent assay (ELISA), blood lymphocyte cell subtypes by flow cytometry, and cytokine mRNA levels by quantitative reverse transcriptase PCR. Group A cats all became infected with high bacterial loads and seroconverted, while group B cats were protected from reinfection, thus providing the unique opportunity to study the immunological parameters associated with this protective immune response against M. haemofelis. First, a strong humoral response to DnaK was only observed in group A, demonstrating that an antibody response to DnaK is not important for protective immunity. Second, proinflammatory cytokine interleukin-6 (IL-6) mRNA levels appeared to increase rapidly postinoculation in group B, indicating a possible role in protective immunity. Third, an increase in IL-12p35 and -p40 mRNA and decrease in the Th2/Th1 ratio observed in group A suggest that a Th1-type response is important in primary infection. This is the first study to demonstrate protective immunity against M. haemofelis reinfection, and it provides important information for potential future hemoplasma vaccine design.
- Language
-
- English
- Open access status
- gold
- Identifiers
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- DOI 10.1128/CVI.00581-14
- PMID 25410206
- Persistent URL
- https://sonar.ch/global/documents/156452
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